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10.1016/j.celrep.2017.08.044 http://scihub22266oqcxt.onion/10.1016/j.celrep.2017.08.044 C5608971!5608971!28903044     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2017 ; 20 (11): 2639-53 Nephropedia Template TP {{tp|p=28903044|t=2017. Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors |pdf=|usr=}}{{28903044}} gab.com Text Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=28903044 #FOAvip Synthetic receptors provide a powerful experimental tool for generation of designer cells capable of monitoring the environment, sensing specific input signals, and executing diverse custom response programs. To advance the promise of cellular engineering, we have developed a class of chimeric receptors that integrate a highly programmable and portable nuclease-deficient CRISPR/Cas9 (dCas9) signal transduction module. We demonstrate that the core dCas9 synthetic receptor (dCas9-synR) architecture can be readily adapted to various classes of native ectodomain scaffolds, linking their natural inputs with orthogonal output functions. Importantly, these receptors achieved stringent OFF/ON state transition characteristics, showed agonist-mediated dose-dependent activation, and could be programmed to couple specific disease markers with diverse, therapeutically relevant multi-gene expression circuits. The modular dCas9-synR platform developed here provides a generalizable blueprint for designing next generations of synthetic receptors, which will enable the implementation of highly complex combinatorial functions in cellular engineering. Twit Text FOAVip Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=28903044 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28903044 #FOAvip English Wikipedia <ref>{{Cite pmid|28903044}}</ref> Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors #MMPMID28903044 Baeumler TA; Ahmed AA; Fulga TA Cell Rep 2017[Sep]; 20 (11): 2639-53 PMID28903044show ga Synthetic receptors provide a powerful experimental tool for generation of designer cells capable of monitoring the environment, sensing specific input signals, and executing diverse custom response programs. To advance the promise of cellular engineering, we have developed a class of chimeric receptors that integrate a highly programmable and portable nuclease-deficient CRISPR/Cas9 (dCas9) signal transduction module. We demonstrate that the core dCas9 synthetic receptor (dCas9-synR) architecture can be readily adapted to various classes of native ectodomain scaffolds, linking their natural inputs with orthogonal output functions. Importantly, these receptors achieved stringent OFF/ON state transition characteristics, showed agonist-mediated dose-dependent activation, and could be programmed to couple specific disease markers with diverse, therapeutically relevant multi-gene expression circuits. The modular dCas9-synR platform developed here provides a generalizable blueprint for designing next generations of synthetic receptors, which will enable the implementation of highly complex combinatorial functions in cellular engineering. äEngineering Synthetic Signaling Pathways with Programmable dCas9-Based(epmc).pdf DeepDyve Pubget Overpricing