Sodium hydrogen sulfide (NaHS) ameliorates alterations caused by cisplatin in
filtration slit diaphragm and podocyte cytoskeletal in rat kidney
#MMPMID29560344
Karimi A
; Absalan F
; Khorsandi L
; Valizadeh A
; Mansouri E
J Nephropathol
2017[Jul]; 6
(3
): 150-156
PMID29560344
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BACKGROUND: Hydrogen sulfide (H2S) has been shown to have a protective role in
various kidney disorders. OBJECTIVES: This study investigated the molecular
mechanism of NaHS (a H2S donor) in treating on the renal damage induced by
cisplatin (CP). MATERIALS AND METHODS: Thirty-two male rats were randomly divided
into 4 groups: Normal control group (group A)' NaHS group (group B) which
received 200 µg/kg/d (intraperitoneal injection; i.p.) for 15 days' CP group
(group C) which rats were injected with CP (5 mg/kg, single dose, i.p.), and CP +
NaHS group (group D) (5 mg/kg and 200 µg/kg, respectively, i.p.). Samples of
urine and serum, tissue of kidney were collected for analysis after treatments
for 15 days. Morphological changes were elevated under light microscope' protein
expression of desmin and nephrin were determined by immunohistochemistry and
western blotting and also malondialdehyde (MDA) level was determined by
spectrophotometer. RESULTS: Compared to the CP group, NaHS treatment mitigated
histological damages, decreased 24-hour urine protein excretion, serum urea and
creatinine as well as MDA level. NaHS treatment increased protein levels of
nephrin. Moreover, NaHS treatment decreased protein levels of desmin.
CONCLUSIONS: NaHS can ameliorate CP -induced renal damage in rats which is
associated with the increase in nephrin protein expression, and the decrease in
MDA level and desmin protein expression.
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