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10.1186/s13059-017-1306-z http://scihub22266oqcxt.onion/10.1186/s13059-017-1306-z C5604343!5604343!28923089     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Biol 2017 ; 18 (ä): ä Nephropedia Template TP {{tp|p=28923089|t=2017. DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A |pdf=|usr=}}{{28923089}} gab.com Text DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=28923089 #FOAvip Background: DNA methylation has widespread effects on gene expression during development. However, our ability to assign specific function to regions of DNA methylation is limited by the poor correlation between global patterns of DNA methylation and gene expression. Results: Here, we utilize nuclease-deactivated Cas9 protein fused to repetitive peptide epitopes (SunTag) recruiting multiple copies of antibody-fused de novo DNA methyltransferase 3A (DNMT3A) (dCas9-SunTag-DNMT3A) to amplify the local DNMT3A concentration to methylate genomic sites of interest. We demonstrate that dCas9-SunTag-DNMT3A dramatically increases CpG methylation at the HOXA5 locus in human embryonic kidney (HEK293T) cells. Furthermore, using a single guide RNA, dCas9-SunTag-DNMT3A is able to methylate a 4.5-kb genomic region and repress HOXA5 gene expression. Reduced representation bisulfite sequencing and RNA-seq show that dCas9-SunTag-DNMT3A methylates regions of interest with minimal impact on the global DNA methylome and transcriptome. Conclusions: This effective and precise tool enables site-specific manipulation of DNA methylation and may be used to address the relationship between DNA methylation and gene expression. Electronic supplementary material: The online version of this article (doi:10.1186/s13059-017-1306-z) contains supplementary material, which is available to authorized users. Twit Text FOAVip DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=28923089 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28923089 #FOAvip English Wikipedia <ref>{{Cite pmid|28923089}}</ref> DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A #MMPMID28923089 Huang YH; Su J; Lei Y; Brunetti L; Gundry MC; Zhang X; Jeong M; Li W; Goodell MA Genome Biol 2017[]; 18 (ä): ä PMID28923089show ga Background: DNA methylation has widespread effects on gene expression during development. However, our ability to assign specific function to regions of DNA methylation is limited by the poor correlation between global patterns of DNA methylation and gene expression. Results: Here, we utilize nuclease-deactivated Cas9 protein fused to repetitive peptide epitopes (SunTag) recruiting multiple copies of antibody-fused de novo DNA methyltransferase 3A (DNMT3A) (dCas9-SunTag-DNMT3A) to amplify the local DNMT3A concentration to methylate genomic sites of interest. We demonstrate that dCas9-SunTag-DNMT3A dramatically increases CpG methylation at the HOXA5 locus in human embryonic kidney (HEK293T) cells. Furthermore, using a single guide RNA, dCas9-SunTag-DNMT3A is able to methylate a 4.5-kb genomic region and repress HOXA5 gene expression. Reduced representation bisulfite sequencing and RNA-seq show that dCas9-SunTag-DNMT3A methylates regions of interest with minimal impact on the global DNA methylome and transcriptome. Conclusions: This effective and precise tool enables site-specific manipulation of DNA methylation and may be used to address the relationship between DNA methylation and gene expression. Electronic supplementary material: The online version of this article (doi:10.1186/s13059-017-1306-z) contains supplementary material, which is available to authorized users. äDNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A (epmc).pdf DeepDyve Pubget Overpricing