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10.1073/pnas.1708304114

http://scihub22266oqcxt.onion/10.1073/pnas.1708304114
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C5604029!5604029!28847959
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suck abstract from ncbi

pmid28847959      Proc+Natl+Acad+Sci+U+S+A 2017 ; 114 (37): 9797-802
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  • Adaptive-illumination STED nanoscopy #MMPMID28847959
  • Heine J; Reuss M; Harke B; D?Este E; Sahl SJ; Hell SW
  • Proc Natl Acad Sci U S A 2017[Sep]; 114 (37): 9797-802 PMID28847959show ga
  • We demonstrate DyMIN (Dynamic Intensity Minimum), a versatile illumination concept for coordinate-targeted superresolution fluorescence imaging. Dynamically adapting the illumination (and therefore resolution) for the probing of molecular signals to local structural features entails major reductions in the light dose applied to the sample over the duration of the image scan. The resulting strong photobleaching reduction benefits signal and image contrast, as well as resolution, in biological nanoscale imaging. We show DyMIN stimulated emission depletion nanoscopy of different challenging samples in two and three dimensions.
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