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10.1038/s41467-017-00606-4 http://scihub22266oqcxt.onion/10.1038/s41467-017-00606-4 C5603529!5603529!28924139     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=28924139|t=2017. SRpHi ratiometric pH biosensors for super-resolution microscopy |pdf=|usr=}}{{28924139}} gab.com Text SRpHi ratiometric pH biosensors for super-resolution microscopy JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28924139 #FOAvip Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250?nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy. Twit Text FOAVip SRpHi ratiometric pH biosensors for super-resolution microscopy ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28924139 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28924139 #FOAvip English Wikipedia <ref>{{Cite pmid|28924139}}</ref> SRpHi ratiometric pH biosensors for super-resolution microscopy #MMPMID28924139 Richardson DS; Gregor C; Winter FR; Urban NT; Sahl SJ; Willig KI; Hell SW Nat Commun 2017[]; 8 (ä): ä PMID28924139show ga Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250?nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy. äSRpHi ratiometric pH biosensors for super-resolution microscopy (epmc).pdf DeepDyve Pubget Overpricing