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10.1186/s13075-017-1418-6 http://scihub22266oqcxt.onion/10.1186/s13075-017-1418-6 C5602958!5602958!28915828     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Arthritis+Res+Ther 2017 ; 19 (ä): ä Nephropedia Template TP {{tp|p=28915828|t=2017. MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis |pdf=|usr=}}{{28915828}} gab.com Text MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis JO: Arthritis Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=28915828 #FOAvip Background: Gout is considered one of the most painful acute conditions caused by deposition of monosodium urate (MSU) crystals within joints. Recent studies have shown that interleukin (IL)-1? is a key inflammatory mediator in acute gouty arthritis (GA), and its level is regulated by microRNAs (miRNAs). However, the molecular mechanisms of the regulation remain unclear. Methods: A miRNA microarray was used to analyze the miRNA expression profiles in peripheral white blood cells (WBCs) of patients with GA. THP-1 cells were transfected with miRNA mimics, stimulated by MSU crystals, and then subjected to quantitative real-time polymerase chain reaction or Western blot analysis. Levels of IL-1?, IL-8, and tumor necrosis factor (TNF)-? in culture supernatants of THP-1 cells were measured by enzyme-linked immunosorbent assay. A luciferase reporter assay was conducted to confirm the interaction of miRNA and IL-1? 3?-untranslated regions (UTRs). Results: Combining bioinformatics and miRNA expression profiles, we found five miRNAs (hsa-miR-30c-1-3p, hsa-miR-488-3p, hsa-miR-550a-3p, hsa-miR-663a, and hsa-miR-920) that possibly target IL-1?. Then, we demonstrated that miR-488 and miR-920 were significantly decreased in the WBCs of patients with GA and that MSU crystals could inhibit expression of miR-488 and miR-920. Upregulation of miR-488 and miR-920 could suppress MSU-induced IL-1? protein expression in THP-1 cells, but no significant difference in IL-1? messenger RNA levels was observed. Moreover, we found that miR-488 and miR-920 could directly target the 3?-UTR of IL-1?. Overexpression of miR-488 and miR-920 could significantly inhibit the gene and protein expression of IL-8 and TNF-? in MSU-induced THP-1 cells. Conclusions: This study demonstrates the roles of miR-488 and miR-920 in regulating the production of proinflammatory cytokines in the pathogenesis of GA. These findings suggest that miR-488 and miR-920 could serve as potential therapeutic targets in the treatment of GA. Electronic supplementary material: The online version of this article (doi:10.1186/s13075-017-1418-6) contains supplementary material, which is available to authorized users. Twit Text FOAVip MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis ????Arthritis Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=28915828 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28915828 #FOAvip English Wikipedia <ref>{{Cite pmid|28915828}}</ref> MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis #MMPMID28915828 Zhou W; Wang Y; Wu R; He Y; Su Q; Shi G Arthritis Res Ther 2017[]; 19 (ä): ä PMID28915828show ga Background: Gout is considered one of the most painful acute conditions caused by deposition of monosodium urate (MSU) crystals within joints. Recent studies have shown that interleukin (IL)-1? is a key inflammatory mediator in acute gouty arthritis (GA), and its level is regulated by microRNAs (miRNAs). However, the molecular mechanisms of the regulation remain unclear. Methods: A miRNA microarray was used to analyze the miRNA expression profiles in peripheral white blood cells (WBCs) of patients with GA. THP-1 cells were transfected with miRNA mimics, stimulated by MSU crystals, and then subjected to quantitative real-time polymerase chain reaction or Western blot analysis. Levels of IL-1?, IL-8, and tumor necrosis factor (TNF)-? in culture supernatants of THP-1 cells were measured by enzyme-linked immunosorbent assay. A luciferase reporter assay was conducted to confirm the interaction of miRNA and IL-1? 3?-untranslated regions (UTRs). Results: Combining bioinformatics and miRNA expression profiles, we found five miRNAs (hsa-miR-30c-1-3p, hsa-miR-488-3p, hsa-miR-550a-3p, hsa-miR-663a, and hsa-miR-920) that possibly target IL-1?. Then, we demonstrated that miR-488 and miR-920 were significantly decreased in the WBCs of patients with GA and that MSU crystals could inhibit expression of miR-488 and miR-920. Upregulation of miR-488 and miR-920 could suppress MSU-induced IL-1? protein expression in THP-1 cells, but no significant difference in IL-1? messenger RNA levels was observed. Moreover, we found that miR-488 and miR-920 could directly target the 3?-UTR of IL-1?. Overexpression of miR-488 and miR-920 could significantly inhibit the gene and protein expression of IL-8 and TNF-? in MSU-induced THP-1 cells. Conclusions: This study demonstrates the roles of miR-488 and miR-920 in regulating the production of proinflammatory cytokines in the pathogenesis of GA. These findings suggest that miR-488 and miR-920 could serve as potential therapeutic targets in the treatment of GA. Electronic supplementary material: The online version of this article (doi:10.1186/s13075-017-1418-6) contains supplementary material, which is available to authorized users. äMicroRNA-488 and -920 regulate the production of proinflammatory cytok(epmc).pdf DeepDyve Pubget Overpricing