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10.21769/BioProtoc.2427 http://scihub22266oqcxt.onion/10.21769/BioProtoc.2427 C5602571!5602571!28932760     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bio+Protoc 2017 ; 7 (15): ä Nephropedia Template TP {{tp|p=28932760|t=2017. Membrane Lipid Screen to Identify Molecular Targets of Biomolecules |pdf=|usr=}}{{28932760}} gab.com Text Membrane Lipid Screen to Identify Molecular Targets of Biomolecules JO: Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28932760 #FOAvip Proteins that bind to and disrupt cell membranes may target specific phospholipids. Here we describe a protocol to identify the lipid targets of proteins and biomolecules. First, we describe a screen to identify lipids in membranes that are specifically bound by the biomolecule of interest. Second, we describe a method for determining if the presence of these lipids within membranes is necessary for membrane disruption. The methods described here were used to determine that the malaria vaccine candidate CelTOS disrupts cell membranes by specifically targeting phosphatidic acid ( Jimah et al., 2016 ). This protocol has a companion protocol: ?Liposome disruption assay to examine lytic properties of biomolecules? which can be applied to examine the ability of the biomolecule to disrupt membranes composed of the lipid target identified by following this protocol ( Jimah et al., 2017 ). Twit Text FOAVip Membrane Lipid Screen to Identify Molecular Targets of Biomolecules ????Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28932760 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28932760 #FOAvip English Wikipedia <ref>{{Cite pmid|28932760}}</ref> Membrane Lipid Screen to Identify Molecular Targets of Biomolecules #MMPMID28932760 Jimah JR; Schlesinger PH; Tolia NH Bio Protoc 2017[Aug]; 7 (15): ä PMID28932760show ga Proteins that bind to and disrupt cell membranes may target specific phospholipids. Here we describe a protocol to identify the lipid targets of proteins and biomolecules. First, we describe a screen to identify lipids in membranes that are specifically bound by the biomolecule of interest. Second, we describe a method for determining if the presence of these lipids within membranes is necessary for membrane disruption. The methods described here were used to determine that the malaria vaccine candidate CelTOS disrupts cell membranes by specifically targeting phosphatidic acid ( Jimah et al., 2016 ). This protocol has a companion protocol: ?Liposome disruption assay to examine lytic properties of biomolecules? which can be applied to examine the ability of the biomolecule to disrupt membranes composed of the lipid target identified by following this protocol ( Jimah et al., 2017 ). äMembrane Lipid Screen to Identify Molecular Targets of Biomolecules (epmc).pdf DeepDyve Pubget Overpricing