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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Biochem+Biophys+Rep
2016 ; 6
(ä): 142-148
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Generation and characterization of monoclonal antibody against Advanced Glycation
End Products in chronic kidney disease
#MMPMID28955871
Finco AB
; Machado-de-Ávila RA
; Maciel R
; De Moura J
; Billiald P
; Stinghen AEM
; Alvarenga LM
Biochem Biophys Rep
2016[Jul]; 6
(ä): 142-148
PMID28955871
show ga
Advanced Glycation End Products (AGEs) are toxins that are involved in structural
and functional alterations of several organs and tissues, resulting in various
pathologies. Several types of AGEs have been described but carboxymethyllysine
(CML) is the major antigenic AGE compound. In this study, three different
immunogenic carrier proteins (KLH, keyhole limpet hemocyanin; BSA, bovine serum
albumin; and HSA, human serum albumin) were modified by glycation. The glycated
molecules were used to produce epitope-specific monoclonal antibodies able to
recognize the CML domain and to detect uremic toxins in the serum of patients
with chronic kidney disease (CKD). A competitive ELISA was standardized in order
to quantify CML in the sera of CKD patients. An increase in uremic toxins can
compromise the clinical condition of these patients, thus, the detection and
quantification of these toxins should contribute to a better management and
understanding of this disease.