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2016 ; 6
(ä): 179-184
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Noncanonical Wnt signaling in stromal cells regulates B-lymphogenesis through
interleukin-7 expression
#MMPMID28955876
Sato M
; Tamura M
Biochem Biophys Rep
2016[Jul]; 6
(ä): 179-184
PMID28955876
show ga
The regulation of early B cell development and the interaction of hematopoietic
precursors with stromal cells in the bone marrow (BM) are controlled by various
secreted signaling molecules. Several recent studies showed Wnt signaling
involved in B-lymphogenesis through stromal cells. However, the molecules
modulated by Wnt signaling in stromal cells regulating B-lymphogenesis have not
been identified yet. Interleukin (IL)-7 and CXC chemokine ligand (CXCL) 12 are
known to be express in stromal cells, and both molecules are essential for
B-lymphogenesis. In the present study, we examined the role of Wnt signaling in
regulating IL-7 and CXCL12 expression and in affecting B-lymphogenesis. In mouse
stromal ST2 cells, expression of IL-7 and CXCL12 mRNA was augmented by
noncanonical Wnt5a. When mouse BM-derived cells were cultured on
Wnt5a-overexpressing ST2 cells, an increased number of B220+/IgM- B-lymphoid
precursor cells was observed. These results show that Wnt5a regulates IL-7 gene
expression in stromal cells and suggest the possibility that noncanonical Wnt
regulates B-lymphogenesis via IL-7 expression in stromal cells.