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2016 ; 5
(ä): 175-179
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Identification of a 48 kDa form of unconventional myosin 1c in blood serum of
patients with autoimmune diseases
#MMPMID28955821
Myronovkij S
; Negrych N
; Nehrych T
; Redowicz MJ
; Souchelnytskyi S
; Stoika R
; Kit Y
Biochem Biophys Rep
2016[Mar]; 5
(ä): 175-179
PMID28955821
show ga
We searched for protein markers present in blood serum of multiple sclerosis
(MS), rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients
in comparison to healthy human individuals. We used precipitation/extraction
methods and MALDI TOF/TOF mass spectrometry, and identified a protein with Mr
~46 kDa as a fragment of human unconventional myosin IC isoform b (Myo1C).
Western blotting with specific anti-human Myo1C antibodies confirmed the
identity. Screening of blood serum samples from different autoimmune patients for
the presence of Myo1c revealed its high level in MS and RA patients, relatively
low level in SLE patients, and undetected in healthy donors. These data are
suggesting that the level of p46 Myo1C in blood serum is a potential marker for
testing of autoimmune diseases.