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10.1021/jacs.7b06555

http://scihub22266oqcxt.onion/10.1021/jacs.7b06555
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C5599110!5599110!28787145
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suck abstract from ncbi


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pmid28787145      J+Am+Chem+Soc 2017 ; 139 (33): 11337-40
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  • Chemically Controlled Epigenome Editing Through an Inducible dCas9 System #MMPMID28787145
  • Chen T; Gao D; Zhang R; Zeng G; Yan H; Lim E; Liang FS
  • J Am Chem Soc 2017[Aug]; 139 (33): 11337-40 PMID28787145show ga
  • Although histone modifications are associated with gene activities, studies of their causal relationships have been difficult. For this purpose, we developed an inducible system integrating dCas9-based targeting and chemically induced proximity technologies to allow small molecule induced recruitment of P300 acetyltransferase and the acetylation of H3K27 at precise gene loci in cells. Employing the new technique, we elucidated the temporal order of histone acetylation and gene activation, as well as the stability of the installed histone modification.
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