Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28910360
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 PLoS+One
2017 ; 12
(9
): e0184738
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Transgelin-2 is upregulated on activated B-cells and expressed in hyperplastic
follicles in lupus erythematosus patients
#MMPMID28910360
Kiso K
; Yoshifuji H
; Oku T
; Hikida M
; Kitagori K
; Hirayama Y
; Nakajima T
; Haga H
; Tsuruyama T
; Miyagawa-Hayashino A
PLoS One
2017[]; 12
(9
): e0184738
PMID28910360
show ga
Transgelin-2 (TAGLN2) is an actin-binding protein that controls actin stability
and promotes T cell activation. TAGLN2 is also expressed on B-cells but its
function in B-cells is unknown. We found that TAGLN2-expressing B-cells were
localized in the germinal center (GC) of secondary lymphoid tissues and TAGLN2
mRNA was significantly upregulated after IgM+IgG stimulation in primary human
B-cells, suggesting that TAGLN2 was upregulated upon B-cell activation. In
support of this, lymph nodes (LNs) from patients with systemic lupus
erythematosus (SLE), in which the intense GC activity have been recognized,
showed increased TAGLN2 expression in B-cells compared to control LNs. Moreover,
TAGLN2+B-cells were distributed widely not only in the GC but also in the
perifollicular areas in SLE LNs. In contrast, CD19+ B-cells and CD19+CD27+
memory-B cells in peripheral blood of SLE patients showed no increase in TAGLN2
mRNA. Two-photon excitation microscopy of Raji cells demonstrated that TAGLN2
colocalized with F-actin and moved together to the periphery upon stimulation.
TAGLN2-knockdown in Raji cells resulted in impaired phosphorylation of PLC?2
leading to inhibition of cell migration. Microarray analysis of TAGLN2-knockdown
Raji cells showed decreased expression of the genes associated with immune
function including CCR6 and as well as of those associated with regulation of the
actin cytoskeleton including ABI2, compared to controls. These results suggest
that TAGLN2 might regulate activation and migration of B-cells, in particular,
the entry of activated B-cells into the follicle. We also suggest that TAGLN2
could be used as a marker for activated B-cells.
free
free
free
