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10.4103/0366-6999.213406 http://scihub22266oqcxt.onion/10.4103/0366-6999.213406 C5598327!5598327 !28836571     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28836571 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Chin+Med+J+(Engl) 2017 ; 130 (18 ): 2163-2169 Nephropedia Template TP {{tp|p=28836571 |t=2017. Pharmacological Inhibition of Macrophage Toll-like Receptor 4/Nuclear Factor-kappa B Alleviates Rhabdomyolysis-induced Acute Kidney Injury |pdf=|usr=}}{{28836571 }} gab.com Text Pharmacological Inhibition of Macrophage Toll-like Receptor 4/Nuclear Factor-kappa B Alleviates Rhabdomyolysis-induced Acute Kidney Injury JO: Chin Med J (Engl) http://www.kidney.de/pget.php?&tw=1&pmid=28836571 #FOAvip BACKGROUND: Acute kidney injury (AKI) is the most common and life-threatening systemic complication of rhabdomyolysis. Inflammation plays an important role in the development of rhabdomyolysis-induced AKI. This study aimed to investigate the kidney model of AKI caused by rhabdomyolysis to verify the role of macrophage Toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-?B) signaling pathway. METHODS: C57BL/6 mice were injected with a 50% glycerin solution at bilateral back limbs to induce rhabdomyolysis, and CLI-095 or pyrrolidine dithiocarbamate (PDTC) was intraperitoneally injected at 0.5 h before molding. Serum creatinine levels, creatine kinase, the expression of tumor necrosis factor (TNF)-?, interleukin (IL)-1? and IL-6, and hematoxylin and eosin stainings of kidney tissues were tested. The infiltration of macrophage, mRNA levels, and protein expression of TLR4 and NF-?B were investigated by immunofluorescence double-staining techniques, reverse transcriptase-quantitative polymerase chain reaction, and Western blotting, respectively. In vitro, macrophage RAW264.7 was stimulated by ferrous myoglobin; the cytokines, TLR4 and NF-?B expressions were also detected. RESULTS: In an in vivo study, using CLI-095 or PDTC to block TLR4/NF-?B, functional and histologic results showed that the inhibition of TLR4 or NF-?B alleviated glycerol-induced renal damages (P < 0.01). CLI-095 or PDTC administration suppressed proinflammatory cytokine (TNF-?, IL-6, and IL-1?) production and macrophage infiltration into the kidney (P < 0.01). Moreover, in an in vitro study, CLI-095 or PDTC suppressed myoglobin-induced expression of TLR4, NF-?B, and proinflammatory cytokine levels in macrophage RAW264.7 cells (P < 0.01). CONCLUSION: The pharmacological inhibition of TLR4/NF-?B exhibited protective effects on rhabdomyolysis-induced AKI by the regulation of proinflammatory cytokine production and macrophage infiltration. Twit Text FOAVip Pharmacological Inhibition of Macrophage Toll-like Receptor 4/Nuclear Factor-kappa B Alleviates Rhabdomyolysis-induced Acute Kidney Injury ????Chin Med J (Engl) http://www.kidney.de/pget.php?&tw=1&pmid=28836571 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28836571 #FOAvip English Wikipedia <ref>{{Cite pmid|28836571 }}</ref> Pharmacological Inhibition of Macrophage Toll-like Receptor 4/Nuclear Factor-kappa B Alleviates Rhabdomyolysis-induced Acute Kidney Injury #MMPMID28836571 Huang RS ; Zhou JJ ; Feng YY ; Shi M ; Guo F ; Gou SJ ; Salerno S ; Ma L ; Fu P Chin Med J (Engl) 2017[Sep]; 130 (18 ): 2163-2169 PMID28836571 show ga BACKGROUND: Acute kidney injury (AKI) is the most common and life-threatening systemic complication of rhabdomyolysis. Inflammation plays an important role in the development of rhabdomyolysis-induced AKI. This study aimed to investigate the kidney model of AKI caused by rhabdomyolysis to verify the role of macrophage Toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-?B) signaling pathway. METHODS: C57BL/6 mice were injected with a 50% glycerin solution at bilateral back limbs to induce rhabdomyolysis, and CLI-095 or pyrrolidine dithiocarbamate (PDTC) was intraperitoneally injected at 0.5 h before molding. Serum creatinine levels, creatine kinase, the expression of tumor necrosis factor (TNF)-?, interleukin (IL)-1? and IL-6, and hematoxylin and eosin stainings of kidney tissues were tested. The infiltration of macrophage, mRNA levels, and protein expression of TLR4 and NF-?B were investigated by immunofluorescence double-staining techniques, reverse transcriptase-quantitative polymerase chain reaction, and Western blotting, respectively. In vitro, macrophage RAW264.7 was stimulated by ferrous myoglobin; the cytokines, TLR4 and NF-?B expressions were also detected. RESULTS: In an in vivo study, using CLI-095 or PDTC to block TLR4/NF-?B, functional and histologic results showed that the inhibition of TLR4 or NF-?B alleviated glycerol-induced renal damages (P < 0.01). CLI-095 or PDTC administration suppressed proinflammatory cytokine (TNF-?, IL-6, and IL-1?) production and macrophage infiltration into the kidney (P < 0.01). Moreover, in an in vitro study, CLI-095 or PDTC suppressed myoglobin-induced expression of TLR4, NF-?B, and proinflammatory cytokine levels in macrophage RAW264.7 cells (P < 0.01). CONCLUSION: The pharmacological inhibition of TLR4/NF-?B exhibited protective effects on rhabdomyolysis-induced AKI by the regulation of proinflammatory cytokine production and macrophage infiltration. |Acute Kidney Injury/*drug therapy/metabolism [MESH] |Animals [MESH] |Blotting, Western [MESH] |Enzyme-Linked Immunosorbent Assay [MESH] |Interleukin-1beta/metabolism [MESH] |Interleukin-6/metabolism [MESH] |Kidney/drug effects/metabolism [MESH] |Macrophages/drug effects/metabolism [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |NF-kappa B/antagonists & inhibitors/*metabolism [MESH] |Proline/analogs & derivatives/pharmacology/therapeutic use [MESH] |Pyrrolidines/pharmacology/therapeutic use [MESH] |RAW 264.7 Cells [MESH] |Rhabdomyolysis/*complications/metabolism [MESH] |Sulfonamides/pharmacology/therapeutic use [MESH] |Thiocarbamates/pharmacology/therapeutic use [MESH] |Toll-Like Receptor 4/antagonists & inhibitors/*metabolism [MESH]Pharmacological Inhibition of Macrophage Toll-like Receptor 4/Nuclear (epmc).pdf DeepDyve Pubget Overpricing