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10.1038/s41598-017-11526-0 http://scihub22266oqcxt.onion/10.1038/s41598-017-11526-0 C5597606!5597606!28904368     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28904368|t=2017. Polycystin-1 inhibits eIF2? phosphorylation and cell apoptosis through a PKR-eIF2? pathway |pdf=|usr=}}{{28904368}} gab.com Text Polycystin-1 inhibits eIF2 phosphorylation and cell apoptosis through a PKR-eIF2 pathway JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28904368 #FOAvip Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations in PKD1 or PKD2 which encodes polycystin-1 (PC1) and polycystin-2, respectively. PC1 was previously shown to slow cell proliferation and inhibit apoptosis but the underlying mechanisms remain elusive or controversial. Here we showed in cultured mammalian cells and Pkd1 knockout mouse kidney epithelial cells that PC1 and its truncation mutant comprising the last five transmembrane segments and the intracellular C-terminus (PC1-5TMC) down-regulate the phosphorylation of protein kinase R (PKR) and its substrate eukaryotic translation initiation factor 2 alpha (eIF2?). PKR is known to be activated by interferons and dsRNAs, inhibits protein synthesis and induces apoptosis. By co-immunoprecipitation experiments we found that PC1 truncation mutants associate with PKR, or with PKR and its activator PACT. Further experiments showed that PC1 and PC1-5TMC reduce phosphorylation of eIF2? through inhibiting PKR phosphorylation. Our TUNEL experiments using tunicamycin, an apoptosis inducer, and GADD34, an inhibitor of eIF2? phosphorylation, demonstrated that PC1-5TMC inhibits apoptosis of HEK293T cells in a PKR-eIF2?-dependent manner, with concurrent up- and down-regulation of Bcl-2 and Bax, respectively, revealed by Western blotting. Involvement of PC1-regulated eIF2? phosphorylation and a PKR-eIF2? pathway in cell apoptosis may be an important part of the mechanism underlying ADPKD pathogenesis. Twit Text FOAVip Polycystin-1 inhibits eIF2 phosphorylation and cell apoptosis through a PKR-eIF2 pathway ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28904368 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28904368 #FOAvip English Wikipedia <ref>{{Cite pmid|28904368}}</ref> Polycystin-1 inhibits eIF2? phosphorylation and cell apoptosis through a PKR-eIF2? pathway #MMPMID28904368 Tang Y; Wang Z; Yang J; Zheng W; Chen D; Wu G; Sandford R; Tang J; Chen XZ Sci Rep 2017[]; 7 (ä): ä PMID28904368show ga Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations in PKD1 or PKD2 which encodes polycystin-1 (PC1) and polycystin-2, respectively. PC1 was previously shown to slow cell proliferation and inhibit apoptosis but the underlying mechanisms remain elusive or controversial. Here we showed in cultured mammalian cells and Pkd1 knockout mouse kidney epithelial cells that PC1 and its truncation mutant comprising the last five transmembrane segments and the intracellular C-terminus (PC1-5TMC) down-regulate the phosphorylation of protein kinase R (PKR) and its substrate eukaryotic translation initiation factor 2 alpha (eIF2?). PKR is known to be activated by interferons and dsRNAs, inhibits protein synthesis and induces apoptosis. By co-immunoprecipitation experiments we found that PC1 truncation mutants associate with PKR, or with PKR and its activator PACT. Further experiments showed that PC1 and PC1-5TMC reduce phosphorylation of eIF2? through inhibiting PKR phosphorylation. Our TUNEL experiments using tunicamycin, an apoptosis inducer, and GADD34, an inhibitor of eIF2? phosphorylation, demonstrated that PC1-5TMC inhibits apoptosis of HEK293T cells in a PKR-eIF2?-dependent manner, with concurrent up- and down-regulation of Bcl-2 and Bax, respectively, revealed by Western blotting. Involvement of PC1-regulated eIF2? phosphorylation and a PKR-eIF2? pathway in cell apoptosis may be an important part of the mechanism underlying ADPKD pathogenesis. äPolycystin-1 inhibits eIF2? phosphorylation and cell apoptosis through(epmc).pdf DeepDyve Pubget Overpricing