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10.1038/s41467-017-00543-2 http://scihub22266oqcxt.onion/10.1038/s41467-017-00543-2 C5596024!5596024 !28900152     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28900152 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2017 ; 8 (1 ): 400 Nephropedia Template TP {{tp|p=28900152 |t=2017. A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation |pdf=|usr=}}{{28900152 }} gab.com Text A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28900152 #FOAvip The ability to create a 3D tissue structure from individual cells and then to stimulate it at will is a major goal for both the biophysics and regenerative medicine communities. Here we show an integrated set of magnetic techniques that meet this challenge using embryonic stem cells (ESCs). We assessed the impact of magnetic nanoparticles internalization on ESCs viability, proliferation, pluripotency and differentiation profiles. We developed magnetic attractors capable of aggregating the cells remotely into a 3D embryoid body. This magnetic approach to embryoid body formation has no discernible impact on ESC differentiation pathways, as compared to the hanging drop method. It is also the base of the final magnetic device, composed of opposing magnetic attractors in order to form embryoid bodies in situ, then stretch them, and mechanically stimulate them at will. These stretched and cyclic purely mechanical stimulations were sufficient to drive ESCs differentiation towards the mesodermal cardiac pathway.The development of embryoid bodies that are responsive to external stimuli is of great interest in tissue engineering. Here, the authors culture embryonic stem cells with magnetic nanoparticles and show that the presence of magnetic fields could affect their aggregation and differentiation. Twit Text FOAVip A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28900152 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28900152 #FOAvip English Wikipedia <ref>{{Cite pmid|28900152 }}</ref> A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation #MMPMID28900152 Du V ; Luciani N ; Richard S ; Mary G ; Gay C ; Mazuel F ; Reffay M ; Menasché P ; Agbulut O ; Wilhelm C Nat Commun 2017[Sep]; 8 (1 ): 400 PMID28900152 show ga The ability to create a 3D tissue structure from individual cells and then to stimulate it at will is a major goal for both the biophysics and regenerative medicine communities. Here we show an integrated set of magnetic techniques that meet this challenge using embryonic stem cells (ESCs). We assessed the impact of magnetic nanoparticles internalization on ESCs viability, proliferation, pluripotency and differentiation profiles. We developed magnetic attractors capable of aggregating the cells remotely into a 3D embryoid body. This magnetic approach to embryoid body formation has no discernible impact on ESC differentiation pathways, as compared to the hanging drop method. It is also the base of the final magnetic device, composed of opposing magnetic attractors in order to form embryoid bodies in situ, then stretch them, and mechanically stimulate them at will. These stretched and cyclic purely mechanical stimulations were sufficient to drive ESCs differentiation towards the mesodermal cardiac pathway.The development of embryoid bodies that are responsive to external stimuli is of great interest in tissue engineering. Here, the authors culture embryonic stem cells with magnetic nanoparticles and show that the presence of magnetic fields could affect their aggregation and differentiation. |Animals [MESH] |Cell Culture Techniques/*instrumentation/*methods [MESH] |Cell Differentiation [MESH] |Cell Survival [MESH] |Embryoid Bodies/cytology [MESH] |Embryonic Stem Cells/*cytology [MESH] |Ferric Compounds/chemistry [MESH] |Magnetics/instrumentation/methods [MESH] |Magnetite Nanoparticles/chemistry [MESH] |Male [MESH]A 3D magnetic tissue stretcher for remote mechanical control of embryo(epmc).pdf DeepDyve Pubget Overpricing