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10.1038/s41598-017-11310-0 http://scihub22266oqcxt.onion/10.1038/s41598-017-11310-0 C5595876!5595876 !28900149     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28900149 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2017 ; 7 (1 ): 11301 Nephropedia Template TP {{tp|p=28900149 |t=2017. Assessment of T-cell receptor repertoire and clonal expansion in peripheral T-cell lymphoma using RNA-seq data |pdf=|usr=}}{{28900149 }} gab.com Text Assessment of T-cell receptor repertoire and clonal expansion in peripheral T-cell lymphoma using RNA-seq data JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28900149 #FOAvip T-cell clonality of peripheral T-cell lymphoma (PTCL) is routinely evaluated with a PCR-based method using genomic DNA. However, there are limitations with this approach. The purpose of this study was to determine the utility of RNA-seq for assessing T-cell clonality and T-cell antigen receptor (TCR) repertoire of the neoplastic T-cells in 108 PTCL samples. TCR transcripts, including complementarity-determining region 3 (CDR3) sequences, were assessed. In normal T cells, the CDR3 sequences were extremely diverse, without any clonotype representing more than 2% of the overall TCR population. Dominant clones could be identified in 65 out of 76 PTCL cases (86%) with adequate TCR transcript expression. In monoclonal cases, the dominant clone varied between 11% and 99% of TCR? transcripts. No unique V? or V? usage was observed. Small T-cell clones were often observed in T- and NK-cell tumors in a percentage higher than observed in reactive conditions. ? chain expression was very low in tumors expressing TCR??, but its expression level was high and clonality was detected in a TCR?? expressing tumor. NK cell lymphoma (NKCL) did not express significant levels of TCR V? or V? genes. RNA-seq is a useful tool for detecting and characterizing clonal TCR rearrangements in PTCL. Twit Text FOAVip Assessment of T-cell receptor repertoire and clonal expansion in peripheral T-cell lymphoma using RNA-seq data ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28900149 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28900149 #FOAvip English Wikipedia <ref>{{Cite pmid|28900149 }}</ref> Assessment of T-cell receptor repertoire and clonal expansion in peripheral T-cell lymphoma using RNA-seq data #MMPMID28900149 Gong Q ; Wang C ; Zhang W ; Iqbal J ; Hu Y ; Greiner TC ; Cornish A ; Kim JH ; Rabadan R ; Abate F ; Wang X ; Inghirami GG ; McKeithan TW ; Chan WC Sci Rep 2017[Sep]; 7 (1 ): 11301 PMID28900149 show ga T-cell clonality of peripheral T-cell lymphoma (PTCL) is routinely evaluated with a PCR-based method using genomic DNA. However, there are limitations with this approach. The purpose of this study was to determine the utility of RNA-seq for assessing T-cell clonality and T-cell antigen receptor (TCR) repertoire of the neoplastic T-cells in 108 PTCL samples. TCR transcripts, including complementarity-determining region 3 (CDR3) sequences, were assessed. In normal T cells, the CDR3 sequences were extremely diverse, without any clonotype representing more than 2% of the overall TCR population. Dominant clones could be identified in 65 out of 76 PTCL cases (86%) with adequate TCR transcript expression. In monoclonal cases, the dominant clone varied between 11% and 99% of TCR? transcripts. No unique V? or V? usage was observed. Small T-cell clones were often observed in T- and NK-cell tumors in a percentage higher than observed in reactive conditions. ? chain expression was very low in tumors expressing TCR??, but its expression level was high and clonality was detected in a TCR?? expressing tumor. NK cell lymphoma (NKCL) did not express significant levels of TCR V? or V? genes. RNA-seq is a useful tool for detecting and characterizing clonal TCR rearrangements in PTCL. |Clonal Evolution/*genetics [MESH] |Computational Biology/methods [MESH] |Gene Expression Profiling [MESH] |High-Throughput Nucleotide Sequencing [MESH] |Humans [MESH] |Lymphoma, T-Cell, Peripheral/*genetics/pathology [MESH] |Mutation [MESH] |Receptors, Antigen, T-Cell/*genetics [MESH] |Sequence Analysis, RNA [MESH]Assessment of T-cell receptor repertoire and clonal expansion in perip(epmc).pdf DeepDyve Pubget Overpricing