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10.1016/j.ymeth.2017.03.020 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2017.03.020 C5595162!5595162 !28351758     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28351758 &cmd=llinks): Failed to open stream: HTTP request failed! 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The mouse cortical meninges are the site of immune responses to many different pathogens, and are accessible to intravital imaging |pdf=|usr=}}{{28351758 }} gab.com Text The mouse cortical meninges are the site of immune responses to many different pathogens, and are accessible to intravital imaging JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=28351758 #FOAvip A wide range of viral and microbial infections are known to cause meningitis, and there is evidence that the meninges are the gateway to pathogenic invasion of the brain parenchyma. Hence observation of these regions has wide application to understanding host-pathogen interactions. Interactions between pathogens and cells of the immune response can be modified by changes in their environment, such as suppression of the flow of blood and lymph, and, particularly in the case of the meninges, with their unsupported membranes, invasive dissection can alter the tissue architecture. For these reasons, intravital imaging through the unperforated skull is the method of choice. We give a protocol for a simple method of two-photon microscopy through the thinned cortical skull of the anesthetized mouse to enable real-time imaging with sub-micron resolution through the meninges and into the superficial brain parenchyma. In reporter mice in which selected cell types express fluorescent proteins, imaging after infection with fluorescent pathogens (lymphocytic choriomeningitis virus, Trypanosoma brucei or Plasmodium berghei) has shown strong recruitment to the cortical meninges of immune cells, including neutrophils, T cells, and putative dendritic cells and macrophages. Without special labeling, the boundaries between the dura mater, the leptomeninx, and the parenchyma are not directly visualized in intravital two-photon microscopy, but other landmarks and characteristics, which we illustrate, allow the researcher to identify the compartment being imaged. While most infectious meningitides are localized mainly in the dura mater, others involve recruitment of immune cells to the leptomeninx. Twit Text FOAVip The mouse cortical meninges are the site of immune responses to many different pathogens, and are accessible to intravital imaging ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=28351758 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28351758 #FOAvip English Wikipedia <ref>{{Cite pmid|28351758 }}</ref> The mouse cortical meninges are the site of immune responses to many different pathogens, and are accessible to intravital imaging #MMPMID28351758 Coles JA ; Stewart-Hutchinson PJ ; Myburgh E ; Brewer JM Methods 2017[Aug]; 127 (ä): 53-61 PMID28351758 show ga A wide range of viral and microbial infections are known to cause meningitis, and there is evidence that the meninges are the gateway to pathogenic invasion of the brain parenchyma. Hence observation of these regions has wide application to understanding host-pathogen interactions. Interactions between pathogens and cells of the immune response can be modified by changes in their environment, such as suppression of the flow of blood and lymph, and, particularly in the case of the meninges, with their unsupported membranes, invasive dissection can alter the tissue architecture. For these reasons, intravital imaging through the unperforated skull is the method of choice. We give a protocol for a simple method of two-photon microscopy through the thinned cortical skull of the anesthetized mouse to enable real-time imaging with sub-micron resolution through the meninges and into the superficial brain parenchyma. In reporter mice in which selected cell types express fluorescent proteins, imaging after infection with fluorescent pathogens (lymphocytic choriomeningitis virus, Trypanosoma brucei or Plasmodium berghei) has shown strong recruitment to the cortical meninges of immune cells, including neutrophils, T cells, and putative dendritic cells and macrophages. Without special labeling, the boundaries between the dura mater, the leptomeninx, and the parenchyma are not directly visualized in intravital two-photon microscopy, but other landmarks and characteristics, which we illustrate, allow the researcher to identify the compartment being imaged. While most infectious meningitides are localized mainly in the dura mater, others involve recruitment of immune cells to the leptomeninx. |*Host-Pathogen Interactions [MESH] |Animals [MESH] |Dendritic Cells [MESH] |Intravital Microscopy/*methods [MESH] |Lymphocytic choriomeningitis virus/physiology [MESH] |Macrophages [MESH] |Meninges/*diagnostic imaging [MESH] |Meningitis/*diagnostic imaging/parasitology/virology [MESH] |Mice [MESH] |Mice, Transgenic [MESH] |Microorganisms, Genetically-Modified [MESH] |Microscopy, Fluorescence, Multiphoton/methods [MESH] |Neutrophils [MESH] |Plasmodium berghei/physiology [MESH] |T-Lymphocytes [MESH]The mouse cortical meninges are the site of immune responses to many d(epmc).pdf DeepDyve Pubget Overpricing