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10.1016/j.actbio.2016.12.033 http://scihub22266oqcxt.onion/10.1016/j.actbio.2016.12.033 C5592694!5592694!27993639     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Acta+Biomater 2017 ; 50 (ä): 207-19 Nephropedia Template TP {{tp|p=27993639|t=2017. The impact of detergents on the tissue decellularization process: a ToF-SIMS study |pdf=|usr=}}{{27993639}} gab.com Text The impact of detergents on the tissue decellularization process: a ToF-SIMS study JO: Acta Biomater http://www.kidney.de/pget.php?&tw=1&pmid=27993639 #FOAvip Biologic scaffolds are derived from mammalian tissues, which must be decellularized to remove cellular antigens that would otherwise incite an adverse immune response. Although widely used clinically, the optimum balance between cell removal and the disruption of matrix architecture and surface ligand landscape remains a considerable challenge. Here we describe the use of time of flight secondary ion mass spectroscopy (ToF-SIMS) to provide sensitive, molecular specific, localized analysis of detergent decellularized biologic scaffolds. We detected residual detergent fragments, specifically from Triton X-100, sodium deoxycholate and sodium dodecyl sulphate (SDS) in decellularized scaffolds; increased SDS concentrations from 0.1% to 1.0% increased both the intensity of SDS fragments and adverse cell outcomes. We also identified cellular remnants, by detecting phosphate and phosphocholine ions in PAA and CHAPS decellularized scaffolds. The present study demonstrates ToF-SIMS is not only a powerful tool for characterization of biologic scaffold surface molecular functionality, but also enables sensitive assessment of decellularization efficacy. Twit Text FOAVip The impact of detergents on the tissue decellularization process: a ToF-SIMS study ????Acta Biomater http://www.kidney.de/pget.php?&tw=1&pmid=27993639 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27993639 #FOAvip English Wikipedia <ref>{{Cite pmid|27993639}}</ref> The impact of detergents on the tissue decellularization process: a ToF-SIMS study #MMPMID27993639 White LJ; Taylor AJ; Faulk DM; Keane TJ; Saldin LT; Reing JE; Swinehart IT; Turner NJ; Ratner BD; Badylak SF Acta Biomater 2017[Mar]; 50 (ä): 207-19 PMID27993639show ga Biologic scaffolds are derived from mammalian tissues, which must be decellularized to remove cellular antigens that would otherwise incite an adverse immune response. Although widely used clinically, the optimum balance between cell removal and the disruption of matrix architecture and surface ligand landscape remains a considerable challenge. Here we describe the use of time of flight secondary ion mass spectroscopy (ToF-SIMS) to provide sensitive, molecular specific, localized analysis of detergent decellularized biologic scaffolds. We detected residual detergent fragments, specifically from Triton X-100, sodium deoxycholate and sodium dodecyl sulphate (SDS) in decellularized scaffolds; increased SDS concentrations from 0.1% to 1.0% increased both the intensity of SDS fragments and adverse cell outcomes. We also identified cellular remnants, by detecting phosphate and phosphocholine ions in PAA and CHAPS decellularized scaffolds. The present study demonstrates ToF-SIMS is not only a powerful tool for characterization of biologic scaffold surface molecular functionality, but also enables sensitive assessment of decellularization efficacy. äThe impact of detergents on the tissue decellularization process: a To(epmc).pdf DeepDyve Pubget Overpricing