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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Int+J+Physiol+Pathophysiol+Pharmacol
2017 ; 9
(4
): 119-126
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The effect of epigallocatechin-3-gallate on the renal dysfunction in the
obstructed kidney in the rat
#MMPMID28951774
Hammad FT
; Lubbad L
Int J Physiol Pathophysiol Pharmacol
2017[]; 9
(4
): 119-126
PMID28951774
show ga
INTRODUCTION: Epigallocatechin-3-gallate (EGCG) is the most active catechin
polyphenol extracted from the green tea. EGCG has protective effects in various
renal and non-renal conditions. The aim of this study was to investigate the
effect of EGCG on the alterations in renal functional parameters following
reversible unilateral ureteral obstruction (UUO) in the rat. METHODS: Wistar rats
underwent reversible left UUO for 72 hours. Group-EGCG (n=10) received
intraperitoneal 50 mg/kg/day of EGCG whereas Group-Vx (n=10) had only normal
saline. Five days post UUO reversal, renal functions of both kidneys were
measured using clearance techniques and the gene expression of some of kidney
injury markers (KIM-1 and NGL) and the pro-inflammatory mediator (TNF-?) were
determined using real time PCR. RESULTS: Renal blood flow, glomerular filtration
rate, urine volume and urinary sodium excretion were still altered 5 days
post-UUO reversal. Fractional sodium excretion had returned to baseline values by
that time. EGCG did not significantly affect any of the renal functional
parameters of the obstructed kidney (P>0.05 for all). However, it significantly
decreased the gene expressions of KIM-1, NGAL and TNF-? in the left obstructed
kidney in Group-EGCG compared to Group-Vx (28±27 vs. 286±107, 1.1±0.2 vs.
10.9±4.3, and 0.8±0.1 vs. 1.5±0.2, P<0.05 for all). CONCLUSION: EGCG appears to
have no significant protective effect on the haemodynamic or tubular glomerular
functions when measured as early as five days post reversal of UUO despite the
attenuation of some of the kidney injury markers and pro-inflammatory mediators.