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10.1186/s40001-017-0272-y http://scihub22266oqcxt.onion/10.1186/s40001-017-0272-y C5591503!5591503!28886732     free     free     free       Eur+J+Med+Res 2017 ; 22 (ä): ä Nephropedia Template TP {{tp|p=28886732|t=2017. Optimized protocol for whole organ decellularization |pdf=|usr=}}{{28886732}} gab.com Text Optimized protocol for whole organ decellularization JO: Eur J Med Res http://www.kidney.de/pget.php?&tw=1&pmid=28886732 #FOAvip Background: The idea of tissue decellularization to gain matrices for tissue engineering is promising. The aim of the present study is to establish a safe and reproducible protocol for solid tissue decellularization that prevents the architecture of the matrix with the inherent vascular network. Methods: The study was performed in rat kidneys which were decellularized by a SDS-based perfusion protocol. Perfusion time and SDS concentration were systematically changed to obtain the shortest and most gentle protocol that leads to complete decellularization. Results: We investigated kinetics of protein elution, decellularization success, and remaining cell toxicity. This resulted in a reproducible protocol, leading to safe decellularization with prevention of the inherent vascular network, without remaining detectable cell toxicity. The established protocol leads to solid tissue decellularization in only 7 h, which is by far shorter than the previously published methods. Conclusion: The established technique has the potential to become a relevant platform technology for tissue engineering of solid tissues. It provides a solution for the yet-unsolved problem of vascularization. Twit Text FOAVip Optimized protocol for whole organ decellularization ????Eur J Med Res http://www.kidney.de/pget.php?&tw=1&pmid=28886732 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28886732 #FOAvip English Wikipedia <ref>{{Cite pmid|28886732}}</ref> Optimized protocol for whole organ decellularization #MMPMID28886732 Schmitt A; Csiki R; Tron A; Saldamli B; Tübel J; Florian K; Siebenlist S; Balmayor E; Burgkart R Eur J Med Res 2017[]; 22 (ä): ä PMID28886732show ga Background: The idea of tissue decellularization to gain matrices for tissue engineering is promising. The aim of the present study is to establish a safe and reproducible protocol for solid tissue decellularization that prevents the architecture of the matrix with the inherent vascular network. Methods: The study was performed in rat kidneys which were decellularized by a SDS-based perfusion protocol. Perfusion time and SDS concentration were systematically changed to obtain the shortest and most gentle protocol that leads to complete decellularization. Results: We investigated kinetics of protein elution, decellularization success, and remaining cell toxicity. This resulted in a reproducible protocol, leading to safe decellularization with prevention of the inherent vascular network, without remaining detectable cell toxicity. The established protocol leads to solid tissue decellularization in only 7 h, which is by far shorter than the previously published methods. Conclusion: The established technique has the potential to become a relevant platform technology for tissue engineering of solid tissues. It provides a solution for the yet-unsolved problem of vascularization. äOptimized protocol for whole organ decellularization (epmc).pdf DeepDyve Pubget Overpricing