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10.14202/vetworld.2017.973-978 http://scihub22266oqcxt.onion/10.14202/vetworld.2017.973-978 C5591488!5591488 !28919692     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28919692 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Vet+World 2017 ; 10 (8 ): 973-978 Nephropedia Template TP {{tp|p=28919692 |t=2017. Isolation and characterization of bacteriophages with lytic activity against common bacterial pathogens |pdf=|usr=}}{{28919692 }} gab.com Text Isolation and characterization of bacteriophages with lytic activity against common bacterial pathogens JO: Vet World http://www.kidney.de/pget.php?&tw=1&pmid=28919692 #FOAvip AIM: Present investigation was conducted to isolate and characterize bacteriophages with lytic activity against common bacterial pathogens. MATERIALS AND METHODS: A total of 60 samples of animal waste disposal from cattle (42) and buffalo (18) farms were collected from three different strata, i.e., top, mid, and bottom of collection tank. Samples were primarily subjected to rapid detection methods, and then isolation of phage was done by double agar layer method using Bacillus subtilis (BsH) and Escherichia coli (EH) as host system. Phages were characterized on the basis of plaque morphology, temperature, pH susceptibility, and host range. RESULTS: Recovery of phages was higher from dairy cattle farm waste (78.57%) as compared to buffalo farm waste (72.22%) and bottom layer of tank showed maximum recovery. Bacillus subtilis (91%) supported the growth of more phages as compared to E. coli (9%). Three different phage morphotypes were observed each against Bacillus subtilis (BsHR(1), BsHR(2), and BsHR(3)) and E. coli (EHR1, EHR(2), and EHR(3)). Mean phage titer of above six phage isolates ranged between 3×10(10) and 5×10(12) plaque forming units/ml. Viability of phages was by, and large unaffected at 70°C within 2-3 min, and phage isolates were completely inactivated below pH 3 and above 11. Coliphage EHR(1) had widest host range followed by BsHR(1) and BsHR(2) while EHR(2), EHR(3), and BsHR(3) had low lytic activity. CONCLUSION: It could be concluded from the present study that the Bacillus and Coli phage has wide host range and thus exhibits the potential to be used as drug substitute tool against common bacterial pathogens. Twit Text FOAVip Isolation and characterization of bacteriophages with lytic activity against common bacterial pathogens ????Vet World http://www.kidney.de/pget.php?&tw=1&pmid=28919692 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28919692 #FOAvip English Wikipedia <ref>{{Cite pmid|28919692 }}</ref> Isolation and characterization of bacteriophages with lytic activity against common bacterial pathogens #MMPMID28919692 Shende RK ; Hirpurkar SD ; Sannat C ; Rawat N ; Pandey V Vet World 2017[Aug]; 10 (8 ): 973-978 PMID28919692 show ga AIM: Present investigation was conducted to isolate and characterize bacteriophages with lytic activity against common bacterial pathogens. MATERIALS AND METHODS: A total of 60 samples of animal waste disposal from cattle (42) and buffalo (18) farms were collected from three different strata, i.e., top, mid, and bottom of collection tank. Samples were primarily subjected to rapid detection methods, and then isolation of phage was done by double agar layer method using Bacillus subtilis (BsH) and Escherichia coli (EH) as host system. Phages were characterized on the basis of plaque morphology, temperature, pH susceptibility, and host range. RESULTS: Recovery of phages was higher from dairy cattle farm waste (78.57%) as compared to buffalo farm waste (72.22%) and bottom layer of tank showed maximum recovery. Bacillus subtilis (91%) supported the growth of more phages as compared to E. coli (9%). Three different phage morphotypes were observed each against Bacillus subtilis (BsHR(1), BsHR(2), and BsHR(3)) and E. coli (EHR1, EHR(2), and EHR(3)). Mean phage titer of above six phage isolates ranged between 3×10(10) and 5×10(12) plaque forming units/ml. Viability of phages was by, and large unaffected at 70°C within 2-3 min, and phage isolates were completely inactivated below pH 3 and above 11. Coliphage EHR(1) had widest host range followed by BsHR(1) and BsHR(2) while EHR(2), EHR(3), and BsHR(3) had low lytic activity. CONCLUSION: It could be concluded from the present study that the Bacillus and Coli phage has wide host range and thus exhibits the potential to be used as drug substitute tool against common bacterial pathogens. äIsolation and characterization of bacteriophages with lytic activity a(epmc).pdf DeepDyve Pubget Overpricing