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10.1038/s41598-017-10704-4 http://scihub22266oqcxt.onion/10.1038/s41598-017-10704-4 C5591254!5591254!28887473     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28887473|t=2017. Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell |pdf=|usr=}}{{28887473}} gab.com Text Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28887473 #FOAvip We demonstrate on-chip, differential DNA and RNA extraction from a single cell using a microfluidic chip and a two-stage lysis protocol. This method enables direct use of the whole extract, without additional washing steps, reducing sample loss. Using this method, the tumor driving pathway in individual cells from a colorectal cancer cell line was determined by applying a Bayesian computational pathway model to sequences obtained from the RNA fraction of a single cell and, the mutations driving the pathway were determined by analyzing sequences obtained from the DNA fraction of the same single cell. This combined functional and mutational pathway assessment of a single cell could be of significant value for dissecting cellular heterogeneity in tumors and analyzing single circulating tumor cells. Twit Text FOAVip Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28887473 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28887473 #FOAvip English Wikipedia <ref>{{Cite pmid|28887473}}</ref> Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell #MMPMID28887473 van Strijp D; Vulders RCM; Larsen NA; Schira J; Baerlocher L; van Driel MA; Pødenphant M; Hansen TS; Kristensen A; Mir KU; Olesen T; Verhaegh WFJ; Marie R; van der Zaag PJ Sci Rep 2017[]; 7 (ä): ä PMID28887473show ga We demonstrate on-chip, differential DNA and RNA extraction from a single cell using a microfluidic chip and a two-stage lysis protocol. This method enables direct use of the whole extract, without additional washing steps, reducing sample loss. Using this method, the tumor driving pathway in individual cells from a colorectal cancer cell line was determined by applying a Bayesian computational pathway model to sequences obtained from the RNA fraction of a single cell and, the mutations driving the pathway were determined by analyzing sequences obtained from the DNA fraction of the same single cell. This combined functional and mutational pathway assessment of a single cell could be of significant value for dissecting cellular heterogeneity in tumors and analyzing single circulating tumor cells. äComplete sequence-based pathway analysis by differential on-chip DNA a(epmc).pdf DeepDyve Pubget Overpricing