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10.2142/biophysico.14.0_127

http://scihub22266oqcxt.onion/10.2142/biophysico.14.0_127
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C5590786!5590786!28900590
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suck abstract from ncbi


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pmid28900590      Biophys+Physicobiol 2017 ; 14 (ä): 127-35
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  • High-speed atomic force microscopy imaging of live mammalian cells #MMPMID28900590
  • Shibata M; Watanabe H; Uchihashi T; Ando T; Yasuda R
  • Biophys Physicobiol 2017[]; 14 (ä): 127-35 PMID28900590show ga
  • Direct imaging of morphological dynamics of live mammalian cells with nanometer resolution under physiological conditions is highly expected, but yet challenging. High-speed atomic force microscopy (HS-AFM) is a unique technique for capturing biomolecules at work under near physiological conditions. However, application of HS-AFM for imaging of live mammalian cells was hard to be accomplished because of collision between a huge mammalian cell and a cantilever during AFM scanning. Here, we review our recent improvements of HS-AFM for imaging of activities of live mammalian cells without significant damage to the cell. The improvement of an extremely long (~3 ?m) AFM tip attached to a cantilever enables us to reduce severe damage to soft mammalian cells. In addition, a combination of HS-AFM with simple fluorescence microscopy allows us to quickly locate the cell in the AFM scanning area. After these improvements, we demonstrate that developed HS-AFM for live mammalian cells is possible to image morphogenesis of filopodia, membrane ruffles, pits open-close formations, and endocytosis in COS-7, HeLa cells as well as hippocampal neurons.
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