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10.1038/s41467-017-00569-6 http://scihub22266oqcxt.onion/10.1038/s41467-017-00569-6 C5589908!5589908 !28883531     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28883531 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2017 ; 8 (1 ): 477 Nephropedia Template TP {{tp|p=28883531 |t=2017. Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions |pdf=|usr=}}{{28883531 }} gab.com Text Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28883531 #FOAvip Protein-based biosensors or activators have been engineered to visualize molecular signals or manipulate cellular functions. Here we integrate these two functionalities into one protein molecule, an integrated sensing and activating protein (iSNAP). A prototype that can detect tyrosine phosphorylation and immediately activate auto-inhibited Shp2 phosphatase, Shp2-iSNAP, is designed through modular assembly. When Shp2-iSNAP is fused to the SIRP? receptor which typically transduces anti-phagocytic signals from the 'don't eat me' CD47 ligand through negative Shp1 signaling, the engineered macrophages not only allow visualization of SIRP? phosphorylation upon CD47 engagement but also rewire the CD47-SIRP? axis into the positive Shp2 signaling, which enhances phagocytosis of opsonized tumor cells. A second SIRP? Syk-iSNAP with redesigned sensor and activator modules can likewise rewire the CD47-SIRP? axis to the pro-phagocytic Syk kinase activation. Thus, our approach can be extended to execute a broad range of sensing and automated reprogramming actions for directed therapeutics.Protein-based biosensors have been engineered to interrogate cellular signaling and manipulate function. Here the authors demonstrate iSNAP, a tool to detect tyrosine phosphorylation and activate desired protein enzymes allowing the control of phagocytosis in macrophages. Twit Text FOAVip Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28883531 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28883531 #FOAvip English Wikipedia <ref>{{Cite pmid|28883531 }}</ref> Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions #MMPMID28883531 Sun J ; Lei L ; Tsai CM ; Wang Y ; Shi Y ; Ouyang M ; Lu S ; Seong J ; Kim TJ ; Wang P ; Huang M ; Xu X ; Nizet V ; Chien S ; Wang Y Nat Commun 2017[Sep]; 8 (1 ): 477 PMID28883531 show ga Protein-based biosensors or activators have been engineered to visualize molecular signals or manipulate cellular functions. Here we integrate these two functionalities into one protein molecule, an integrated sensing and activating protein (iSNAP). A prototype that can detect tyrosine phosphorylation and immediately activate auto-inhibited Shp2 phosphatase, Shp2-iSNAP, is designed through modular assembly. When Shp2-iSNAP is fused to the SIRP? receptor which typically transduces anti-phagocytic signals from the 'don't eat me' CD47 ligand through negative Shp1 signaling, the engineered macrophages not only allow visualization of SIRP? phosphorylation upon CD47 engagement but also rewire the CD47-SIRP? axis into the positive Shp2 signaling, which enhances phagocytosis of opsonized tumor cells. A second SIRP? Syk-iSNAP with redesigned sensor and activator modules can likewise rewire the CD47-SIRP? axis to the pro-phagocytic Syk kinase activation. Thus, our approach can be extended to execute a broad range of sensing and automated reprogramming actions for directed therapeutics.Protein-based biosensors have been engineered to interrogate cellular signaling and manipulate function. Here the authors demonstrate iSNAP, a tool to detect tyrosine phosphorylation and activate desired protein enzymes allowing the control of phagocytosis in macrophages. |Animals [MESH] |Antigens, Differentiation/genetics/metabolism [MESH] |CD47 Antigen/genetics/*metabolism [MESH] |Cells, Cultured [MESH] |Female [MESH] |Fibroblasts/metabolism [MESH] |Fluorescence Resonance Energy Transfer [MESH] |Humans [MESH] |Macrophages/*metabolism [MESH] |Mice, Inbred C57BL [MESH] |Phagocytosis [MESH] |Phosphorylation [MESH] |Protein Engineering/*methods [MESH] |Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics/metabolism [MESH] |Receptors, Immunologic/genetics/metabolism [MESH] |Recombinant Proteins/genetics/*metabolism [MESH] |Signal Transduction [MESH] |Syk Kinase/genetics/metabolism [MESH]Engineered proteins with sensing and activating modules for automated (epmc).pdf DeepDyve Pubget Overpricing