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10.1016/j.cell.2016.08.050

http://scihub22266oqcxt.onion/10.1016/j.cell.2016.08.050
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C5589196!5589196!27610567
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suck abstract from ncbi


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pmid27610567      Cell 2016 ; 166 (6): 1411-1422.e16
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  • Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex #MMPMID27610567
  • Robinson PJ; Trnka MJ; Bushnell DA; Davis R; Mattei PJ; Burlingame AL; Kornberg RD
  • Cell 2016[Sep]; 166 (6): 1411-1422.e16 PMID27610567show ga
  • A complete, 52-protein, 2.5 million Dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.
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