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2017 ; 14
(3
): 2926-2932
Nephropedia Template TP
gab.com Text
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English Wikipedia
Celastrus orbiculatus extract suppresses the epithelial-mesenchymal transition by
mediating cytoskeleton rearrangement via inhibition of the Cofilin 1 signaling
pathway in human gastric cancer
#MMPMID28927046
Wang H
; Gu H
; Feng J
; Qian Y
; Yang L
; Jin F
; Wang X
; Chen J
; Shi Y
; Lu S
; Zhao M
; Liu Y
Oncol Lett
2017[Sep]; 14
(3
): 2926-2932
PMID28927046
show ga
Celastrus orbiculatus is a traditional medicinal plant used in the
anti-inflammatory and analgesic treatment of various diseases. A previous study
demonstrated that ethyl acetate extract of C. orbiculatus (COE) exhibited
significant antitumor effects. However, studies concerning the effects and
mechanism of COE in terms of suppressing the epithelial-mesenchymal transition
(EMT) in human gastric adenocarcinoma cells have not been performed at present.
The present study hypothesized that COE may inhibit EMT in gastric adenocarcinoma
cells by regulating cell cytoskeleton rearrangement. The effect of COE on the
viability of AGS cells was detected by MTT assay. An EMT model was induced by
transforming growth factor-?1. Cell cytoskeleton staining, laser scanning
confocal microscopy and electronic microscopy were used to detect the changes in
cell morphology and microstructure of gastric adenocarcinoma cells prior and
subsequent to COE treatment. Invasion and migration assays were used to observe
the effect of COE on the metastatic ability of AGS cells in vitro. The effect of
COE on the expression of Cofilin 1 and EMT biomarkers, including
Epithelial-cadherin, Neural-cadherin, Vimentin and matrix metalloproteinases, was
examined by western blotting in AGS cells. The correlation between Cofilin 1 and
EMT was investigated with immunofluorescence and cytoskeleton staining methods.
The results demonstrated that COE may significantly inhibit the process of EMT in
AGS cells, and that this was concentration-dependent. In addition, COE
significantly downregulated the level of Cofilin 1 in a concentration-dependent
manner. In conclusion, these results suggested that Cofilin 1 was directly
involved in the process of EMT in AGS cells, and that it served an important
function. COE may significantly inhibit EMT in AGS cells, potentially by
inhibiting the activation of the Cofilin 1 signaling pathway. The present study
may provide a basis for the development of novel anticancer drugs and the
development of novel therapeutic strategies, targeting Cofilin 1 protein.
free
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