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2017 ; 14
(3
): 3313-3318
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Identification of target gene of venous thromboembolism in patients with lymphoma
via microarray analysis
#MMPMID28927082
Liu P
; Jiang W
; Zhang H
Oncol Lett
2017[Sep]; 14
(3
): 3313-3318
PMID28927082
show ga
Patients with lymphoma are at high risk of developing venous thromboembolism
(VTE). The purpose of the present study was to identify the target gene
associated with VTE for patients with lymphoma. Microarray data was downloaded
from the gene expression omnibus database (GSE17078), which comprised the control
group, 27 normal blood outgrowth endothelial cell (BOEC) samples, and the case
group, 3 BOEC samples of venous thrombosis with protein C deficiency.
Differentially expressed genes (DEGs) were identified by the Limma package of R.
Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway
analyses were performed via the database for annotation, visualization and
integrated discovery. Differentially coexpressed pairs were identified by the
DCGL package of R. The subsequent protein-protein interaction (PPI) networks and
gene coexpression networks were constructed by the Search Tool for the Retrieval
of Interacting Genes/Proteins database, and were visualized by Cytoscape
software. A total of 110 DEGs were obtained, including 73 upregulated and 37
downregulated genes. GO and KEGG pathway enrichment analyses identified 132
significant GO terms and 9 significant KEGG pathways. In total, 97 PPI pairs for
PPI network and 309 differential coexpression pairs for the gene coexpression
network were obtained. Additionally, the connective tissue growth factor (CTGF)
gene was closely connected with other genes in the two networks. A total of 2
KEGG pathways were associated with VTE and CTGF may be the target gene of VTE in
patients with lymphoma. The present study may identify the molecular mechanism of
VTE, but additional clinical study is required to validate the results.