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10.1093/nar/gkx523 http://scihub22266oqcxt.onion/10.1093/nar/gkx523 C5587751!5587751 !28605514     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28605514 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nucleic+Acids+Res 2017 ; 45 (15 ): 8835-8843 Nephropedia Template TP {{tp|p=28605514 |t=2017. Flipping states: a few key residues decide the winning conformation of the only universally conserved transcription factor |pdf=|usr=}}{{28605514 }} gab.com Text Flipping states: a few key residues decide the winning conformation of the only universally conserved transcription factor JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28605514 #FOAvip Transcription factors from the NusG family bind to the elongating RNA polymerase to enable synthesis of long RNAs in all domains of life. In bacteria, NusG frequently co-exists with specialized paralogs that regulate expression of a small set of targets, many of which encode virulence factors. Escherichia coli RfaH is the exemplar of this regulatory mechanism. In contrast to NusG, which freely binds to RNA polymerase, RfaH exists in a structurally distinct autoinhibitory state in which the RNA polymerase-binding site is buried at the interface between two RfaH domains. Binding to an ops DNA sequence triggers structural transformation wherein the domains dissociate and RfaH refolds into a NusG-like structure. Formation of the autoinhibitory state, and thus sequence-specific recruitment, represents the decisive step in the evolutionary history of the RfaH subfamily. We used computational and experimental approaches to identify the residues that confer the unique regulatory properties of RfaH. Our analysis highlighted highly conserved Ile and Phe residues at the RfaH interdomain interface. Replacement of these residues with equally conserved Glu and Val counterpart residues in NusG destabilized interactions between the RfaH domains and allowed sequence-independent recruitment to RNA polymerase, suggesting a plausible pathway for diversification of NusG paralogs. Twit Text FOAVip Flipping states: a few key residues decide the winning conformation of the only universally conserved transcription factor ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=28605514 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28605514 #FOAvip English Wikipedia <ref>{{Cite pmid|28605514 }}</ref> Flipping states: a few key residues decide the winning conformation of the only universally conserved transcription factor #MMPMID28605514 Shi D ; Svetlov D ; Abagyan R ; Artsimovitch I Nucleic Acids Res 2017[Sep]; 45 (15 ): 8835-8843 PMID28605514 show ga Transcription factors from the NusG family bind to the elongating RNA polymerase to enable synthesis of long RNAs in all domains of life. In bacteria, NusG frequently co-exists with specialized paralogs that regulate expression of a small set of targets, many of which encode virulence factors. Escherichia coli RfaH is the exemplar of this regulatory mechanism. In contrast to NusG, which freely binds to RNA polymerase, RfaH exists in a structurally distinct autoinhibitory state in which the RNA polymerase-binding site is buried at the interface between two RfaH domains. Binding to an ops DNA sequence triggers structural transformation wherein the domains dissociate and RfaH refolds into a NusG-like structure. Formation of the autoinhibitory state, and thus sequence-specific recruitment, represents the decisive step in the evolutionary history of the RfaH subfamily. We used computational and experimental approaches to identify the residues that confer the unique regulatory properties of RfaH. Our analysis highlighted highly conserved Ile and Phe residues at the RfaH interdomain interface. Replacement of these residues with equally conserved Glu and Val counterpart residues in NusG destabilized interactions between the RfaH domains and allowed sequence-independent recruitment to RNA polymerase, suggesting a plausible pathway for diversification of NusG paralogs. |*Gene Expression Regulation, Bacterial [MESH] |Amino Acid Sequence [MESH] |Amino Acid Substitution [MESH] |Binding Sites [MESH] |DNA, Bacterial/*chemistry/genetics/metabolism [MESH] |DNA-Directed RNA Polymerases/*chemistry/genetics/metabolism [MESH] |Escherichia coli Proteins/*chemistry/genetics/metabolism [MESH] |Escherichia coli/genetics/metabolism [MESH] |Evolution, Molecular [MESH] |Glutamic Acid/chemistry/metabolism [MESH] |Isoleucine/chemistry/metabolism [MESH] |Models, Molecular [MESH] |Peptide Elongation Factors/*chemistry/genetics/metabolism [MESH] |Phenylalanine/chemistry/metabolism [MESH] |Protein Binding [MESH] |Protein Folding [MESH] |Protein Interaction Domains and Motifs [MESH] |Sequence Alignment [MESH] |Sequence Homology, Amino Acid [MESH] |Trans-Activators/*chemistry/genetics/metabolism [MESH] |Transcription Factors/*chemistry/genetics/metabolism [MESH] |Transcription, Genetic [MESH]Flipping states: a few key residues decide the winning conformation of(epmc).pdf DeepDyve Pubget Overpricing