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10.1038/s41598-017-10479-8 http://scihub22266oqcxt.onion/10.1038/s41598-017-10479-8 C5587737!5587737 !28878382     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28878382 &cmd=llinks): Failed to open stream: HTTP request failed! 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Pleomorphic bacteria-like structures in human blood represent non-living membrane vesicles and protein particles |pdf=|usr=}}{{28878382 }} gab.com Text Pleomorphic bacteria-like structures in human blood represent non-living membrane vesicles and protein particles JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28878382 #FOAvip Although human blood is believed to be a sterile environment, recent studies suggest that pleomorphic bacteria exist in the blood of healthy humans. These studies have led to the development of "live-blood analysis," a technique used by alternative medicine practitioners to diagnose various human conditions, including allergies, cancer, cardiovascular disease and septicemia. We show here that bacteria-like vesicles and refringent particles form in healthy human blood observed under dark-field microscopy. These structures gradually increase in number during incubation and show morphologies reminiscent of cells undergoing division. Based on lipid analysis and Western blotting, we show that the bacteria-like entities consist of membrane vesicles containing serum and exosome proteins, including albumin, fetuin-A, apolipoprotein-A1, alkaline phosphatase, TNFR1 and CD63. In contrast, the refringent particles represent protein aggregates that contain several blood proteins. 16S rDNA PCR analysis reveals the presence of bacterial DNA in incubated blood samples but also in negative controls, indicating that the amplified sequences represent contaminants. These results suggest that the bacteria-like vesicles and refringent particles observed in human blood represent non-living membrane vesicles and protein aggregates derived from blood. The phenomena observed during live-blood analysis are therefore consistent with time-dependent decay of cells and body fluids during incubation ex vivo. Twit Text FOAVip Pleomorphic bacteria-like structures in human blood represent non-living membrane vesicles and protein particles ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28878382 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28878382 #FOAvip English Wikipedia <ref>{{Cite pmid|28878382 }}</ref> Pleomorphic bacteria-like structures in human blood represent non-living membrane vesicles and protein particles #MMPMID28878382 Martel J ; Wu CY ; Huang PR ; Cheng WY ; Young JD Sci Rep 2017[Sep]; 7 (1 ): 10650 PMID28878382 show ga Although human blood is believed to be a sterile environment, recent studies suggest that pleomorphic bacteria exist in the blood of healthy humans. These studies have led to the development of "live-blood analysis," a technique used by alternative medicine practitioners to diagnose various human conditions, including allergies, cancer, cardiovascular disease and septicemia. We show here that bacteria-like vesicles and refringent particles form in healthy human blood observed under dark-field microscopy. These structures gradually increase in number during incubation and show morphologies reminiscent of cells undergoing division. Based on lipid analysis and Western blotting, we show that the bacteria-like entities consist of membrane vesicles containing serum and exosome proteins, including albumin, fetuin-A, apolipoprotein-A1, alkaline phosphatase, TNFR1 and CD63. In contrast, the refringent particles represent protein aggregates that contain several blood proteins. 16S rDNA PCR analysis reveals the presence of bacterial DNA in incubated blood samples but also in negative controls, indicating that the amplified sequences represent contaminants. These results suggest that the bacteria-like vesicles and refringent particles observed in human blood represent non-living membrane vesicles and protein aggregates derived from blood. The phenomena observed during live-blood analysis are therefore consistent with time-dependent decay of cells and body fluids during incubation ex vivo. |*Blood Proteins [MESH] |Biomarkers [MESH] |Dynamic Light Scattering [MESH] |Extracellular Vesicles/*metabolism/ultrastructure [MESH] |Humans [MESH] |Lipids/blood [MESH] |Microscopy [MESH] |Protein Aggregates [MESH] |Proteins/*metabolism [MESH] |RNA, Ribosomal, 16S/genetics [MESH]Pleomorphic bacteria-like structures in human blood represent non-livi(epmc).pdf DeepDyve Pubget Overpricing