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10.1161/JAHA.117.005537 http://scihub22266oqcxt.onion/10.1161/JAHA.117.005537 C5586285!5586285 !28698260     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28698260 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Am+Heart+Assoc 2017 ; 6 (7 ): ä Nephropedia Template TP {{tp|p=28698260 |t=2017. Phospholipase C?1 Mediates Intima Formation Through Akt-Notch1 Signaling Independent of the Phospholipase Activity |pdf=|usr=}}{{28698260 }} gab.com Text Phospholipase C 1 Mediates Intima Formation Through Akt-Notch1 Signaling Independent of the Phospholipase Activity JO: J Am Heart Assoc http://www.kidney.de/pget.php?&tw=1&pmid=28698260 #FOAvip BACKGROUND: Vascular smooth muscle cell proliferation, migration, and dedifferentiation are critical for vascular diseases. Recently, it was demonstrated that Notch receptors have opposing effects on intima formation after vessel injury. Therefore, it is important to investigate the specific regulatory pathways that activate the different Notch receptors. METHODS AND RESULTS: There was a time- and dose-dependent activation of Notch1 by angiotensin II and platelet-derived growth factor in vascular smooth muscle cells. When phospholipase C?1 (PLC?1) expression was reduced by small interfering RNA, Notch1 activation and Hey2 expression (Notch target gene) induced by angiotensin II or platelet-derived growth factor were remarkably inhibited, while Notch2 degradation was not affected. Mechanistically, we observed an association of PLC?1 and Akt, which increased after angiotensin II or platelet-derived growth factor stimulation. PLC?1 knockdown significantly inhibited Akt activation. Importantly, PLC?1 phospholipase site mutation (no phospholipase activity) did not affect Akt activation. Furthermore, PLC?1 depletion inhibited platelet-derived growth factor-induced vascular smooth muscle cell proliferation, migration, and dedifferentiation, while it increased apoptosis. In vivo, PLC?1 and control small interfering RNA were delivered periadventitially in pluronic gel and complete carotid artery ligation was performed. Morphometric analysis 21 days after ligation demonstrated that PLC?1 small interfering RNA robustly attenuated intima area and intima/media ratio compared with the control group. CONCLUSIONS: PLC?1-Akt-mediated Notch1 signaling is crucial for intima formation. This effect is attributable to PLC?1-Akt interaction but not PLC?1 phospholipase activity. Specific inhibition of the PLC?1 and Akt interaction will be a promising therapeutic strategy for preventing vascular remodeling. Twit Text FOAVip Phospholipase C 1 Mediates Intima Formation Through Akt-Notch1 Signaling Independent of the Phospholipase Activity ????J Am Heart Assoc http://www.kidney.de/pget.php?&tw=1&pmid=28698260 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28698260 #FOAvip English Wikipedia <ref>{{Cite pmid|28698260 }}</ref> Phospholipase C?1 Mediates Intima Formation Through Akt-Notch1 Signaling Independent of the Phospholipase Activity #MMPMID28698260 Jiang D ; Zhuang J ; Peng W ; Lu Y ; Liu H ; Zhao Q ; Chi C ; Li X ; Zhu G ; Xu X ; Yan C ; Xu Y ; Ge J ; Pang J J Am Heart Assoc 2017[Jul]; 6 (7 ): ä PMID28698260 show ga BACKGROUND: Vascular smooth muscle cell proliferation, migration, and dedifferentiation are critical for vascular diseases. Recently, it was demonstrated that Notch receptors have opposing effects on intima formation after vessel injury. Therefore, it is important to investigate the specific regulatory pathways that activate the different Notch receptors. METHODS AND RESULTS: There was a time- and dose-dependent activation of Notch1 by angiotensin II and platelet-derived growth factor in vascular smooth muscle cells. When phospholipase C?1 (PLC?1) expression was reduced by small interfering RNA, Notch1 activation and Hey2 expression (Notch target gene) induced by angiotensin II or platelet-derived growth factor were remarkably inhibited, while Notch2 degradation was not affected. Mechanistically, we observed an association of PLC?1 and Akt, which increased after angiotensin II or platelet-derived growth factor stimulation. PLC?1 knockdown significantly inhibited Akt activation. Importantly, PLC?1 phospholipase site mutation (no phospholipase activity) did not affect Akt activation. Furthermore, PLC?1 depletion inhibited platelet-derived growth factor-induced vascular smooth muscle cell proliferation, migration, and dedifferentiation, while it increased apoptosis. In vivo, PLC?1 and control small interfering RNA were delivered periadventitially in pluronic gel and complete carotid artery ligation was performed. Morphometric analysis 21 days after ligation demonstrated that PLC?1 small interfering RNA robustly attenuated intima area and intima/media ratio compared with the control group. CONCLUSIONS: PLC?1-Akt-mediated Notch1 signaling is crucial for intima formation. This effect is attributable to PLC?1-Akt interaction but not PLC?1 phospholipase activity. Specific inhibition of the PLC?1 and Akt interaction will be a promising therapeutic strategy for preventing vascular remodeling. |*Neointima [MESH] |*Signal Transduction/drug effects [MESH] |Angiotensin II/pharmacology [MESH] |Animals [MESH] |Apoptosis [MESH] |Carotid Artery Injuries/*enzymology/genetics/pathology [MESH] |Cell Dedifferentiation [MESH] |Cell Movement [MESH] |Cell Proliferation [MESH] |Cells, Cultured [MESH] |Disease Models, Animal [MESH] |Dose-Response Relationship, Drug [MESH] |Male [MESH] |Mice, Inbred C57BL [MESH] |Muscle, Smooth, Vascular/drug effects/*enzymology/pathology [MESH] |Myocytes, Smooth Muscle/drug effects/*enzymology/pathology [MESH] |Phospholipase C gamma/genetics/*metabolism [MESH] |Phosphorylation [MESH] |Platelet-Derived Growth Factor/pharmacology [MESH] |Proto-Oncogene Proteins c-akt/*metabolism [MESH] |RNA Interference [MESH] |Rats [MESH] |Receptor, Notch1/*metabolism [MESH] |Time Factors [MESH] |Transfection [MESH]Phospholipase C?1 Mediates Intima Formation Through Akt-Notch1 Signali(epmc).pdf DeepDyve Pubget Overpricing