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2017 ; 14
(4
): 3309-3313
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Atorvastatin alleviates iodinated contrast media-induced cytotoxicity in human
proximal renal tubular epithelial cells
#MMPMID28912882
Liu GL
; Lei R
; Duan SB
; Tang MM
; Luo M
; Xu Q
Exp Ther Med
2017[Oct]; 14
(4
): 3309-3313
PMID28912882
show ga
Contrast media (CM)-induced nephropathy (CIN) is a serious complication of
intravascularly applied radiocontrast media. At present, no drugs have been
approved for the prevention of CIN. The present study aimed to explore the
effects and potential mechanisms of atorvastatin on iodinated CM-induced
cytotoxicity in the human proximal renal tubular epithelial cells. The cytotoxic
effect of iohexol (50, 100 and 200 mg I/ml) and the protective effect of
atorvastatin pretreatment (1, 20 and 40 µM) were assessed. The cytotoxicity of
iohexol was evaluated via the MTT cell viability and lactate dehydrogenase
assays. The amount of apoptotic cells was determined by flow cytometry.
Morphological changes in HK-2 cells were observed via transmission electron
microscopy. The mRNA expression of NOX4 and p22phox was measured through reverse
transcription-quantitative polymerase chain reaction analysis. The cytotoxicity
was induced by iohexol in HK-2 cells. Atorvastatin was identified to
significantly alleviate the suppression of cell viability induced by iohexol.
Notably, 40 µM atorvastatin also significantly reduced the mRNA expression of
intracellular NOX4 and p22phox, and the percentage of apoptotic cells.
Furthermore, morphological changes characteristic of injured cells were
alleviated by atorvastatin pretreatment. These results suggest that atorvastatin
exhibits a protective effect on HK-2 cells against iohexol-induced cytotoxicity
through the downregulation of NOX4 and p22phox. Thus, atorvastatin is a potential
therapeutic agent for the prevention of CIN and required further study.
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