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10.1371/journal.pone.0183972 http://scihub22266oqcxt.onion/10.1371/journal.pone.0183972 C5584923!5584923 !28873461     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28873461 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2017 ; 12 (9 ): e0183972 Nephropedia Template TP {{tp|p=28873461 |t=2017. Interleukin-17 induces human alveolar epithelial to mesenchymal cell transition via the TGF-?1 mediated Smad2/3 and ERK1/2 activation |pdf=|usr=}}{{28873461 }} gab.com Text Interleukin-17 induces human alveolar epithelial to mesenchymal cell transition via the TGF- 1 mediated Smad2/3 and ERK1/2 activation JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28873461 #FOAvip Idiopathic pulmonary fibrosis (IPF) is a chronic and usually progressive lung disease and the epithelial-mesenchymal transition (EMT) may play an important role in the pathogenesis of pulmonary fibrosis. IL-17 is a proinflammatory cytokine which promotes EMT profiles in lung inflammatory diseases. In this study, we investigated the effect of IL-17 on EMT in alveolar epithelial cell line A549 and the role of TGF?1-Smad and ERK signaling pathways in the process. Morphological observation on the cells was performed under inverted microscope. The mRNA and protein expressions of E-cad and ?-SMA were detected by quantitative RT-PCR and western blotting. The mRNA and protein expressions of TGF-?1 were analyzed via quantitative RT-PCR and ELISA. Expressions of Smad2/3, p-Smad2/3, ERK1/2, p-ERK1/2 and p-JNK were examined by western blotting. The results indicated that IL-17 can induce A549 cells to undergo morphological changes and phenotypic markers changes, such as down-regulated E-cad expression and up-regulated ?-SMA expression. Additionally, IL-17 enhanced TGF-?1 expression and stimulated Smad2/3 and ERK1/2 phosphorylation in A549 cells. However, there were no significant differences in the expression of phosphorylated JNK in A549 cells with or without IL-17 treatment. SB431542 or U0126 treated cells showed inhibited morphological changes and phenotypic markers expression, such as up-regulated E-cad expression and down-regulated ?-SMA expression. In summary, our results suggest that IL-17 can induce A549 alveolar epithelial cells to undergo EMT via the TGF-?1 mediated Smad2/3 and ERK1/2 activation. Twit Text FOAVip Interleukin-17 induces human alveolar epithelial to mesenchymal cell transition via the TGF- 1 mediated Smad2/3 and ERK1/2 activation ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28873461 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28873461 #FOAvip English Wikipedia <ref>{{Cite pmid|28873461 }}</ref> Interleukin-17 induces human alveolar epithelial to mesenchymal cell transition via the TGF-?1 mediated Smad2/3 and ERK1/2 activation #MMPMID28873461 Wang T ; Liu Y ; Zou JF ; Cheng ZS PLoS One 2017[]; 12 (9 ): e0183972 PMID28873461 show ga Idiopathic pulmonary fibrosis (IPF) is a chronic and usually progressive lung disease and the epithelial-mesenchymal transition (EMT) may play an important role in the pathogenesis of pulmonary fibrosis. IL-17 is a proinflammatory cytokine which promotes EMT profiles in lung inflammatory diseases. In this study, we investigated the effect of IL-17 on EMT in alveolar epithelial cell line A549 and the role of TGF?1-Smad and ERK signaling pathways in the process. Morphological observation on the cells was performed under inverted microscope. The mRNA and protein expressions of E-cad and ?-SMA were detected by quantitative RT-PCR and western blotting. The mRNA and protein expressions of TGF-?1 were analyzed via quantitative RT-PCR and ELISA. Expressions of Smad2/3, p-Smad2/3, ERK1/2, p-ERK1/2 and p-JNK were examined by western blotting. The results indicated that IL-17 can induce A549 cells to undergo morphological changes and phenotypic markers changes, such as down-regulated E-cad expression and up-regulated ?-SMA expression. Additionally, IL-17 enhanced TGF-?1 expression and stimulated Smad2/3 and ERK1/2 phosphorylation in A549 cells. However, there were no significant differences in the expression of phosphorylated JNK in A549 cells with or without IL-17 treatment. SB431542 or U0126 treated cells showed inhibited morphological changes and phenotypic markers expression, such as up-regulated E-cad expression and down-regulated ?-SMA expression. In summary, our results suggest that IL-17 can induce A549 alveolar epithelial cells to undergo EMT via the TGF-?1 mediated Smad2/3 and ERK1/2 activation. |A549 Cells [MESH] |Epithelial Cells/*cytology [MESH] |Epithelial-Mesenchymal Transition/drug effects [MESH] |Extracellular Signal-Regulated MAP Kinases/metabolism [MESH] |Humans [MESH] |Interleukin-17/*metabolism [MESH] |MAP Kinase Kinase 4/metabolism [MESH] |Mesenchymal Stem Cells/*cytology [MESH] |Phosphorylation [MESH] |Pulmonary Alveoli/*cytology [MESH] |Pulmonary Fibrosis/metabolism [MESH] |RNA, Messenger/metabolism [MESH] |Smad2 Protein/metabolism [MESH] |Smad3 Protein/*metabolism [MESH]Interleukin-17 induces human alveolar epithelial to mesenchymal cell t(epmc).pdf DeepDyve Pubget Overpricing