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10.1371/journal.pone.0184281 http://scihub22266oqcxt.onion/10.1371/journal.pone.0184281 C5584922!5584922!28873439     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2017 ; 12 (9): ä Nephropedia Template TP {{tp|p=28873439|t=2017. CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments |pdf=|usr=}}{{28873439}} gab.com Text CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28873439 #FOAvip The recently developed CRISPR screen technology, based on the CRISPR/Cas9 genome editing system, enables genome-wide interrogation of gene functions in an efficient and cost-effective manner. Although many computational algorithms and web servers have been developed to design single-guide RNAs (sgRNAs) with high specificity and efficiency, algorithms specifically designed for conducting CRISPR screens are still lacking. Here we present CRISPR-FOCUS, a web-based platform to search and prioritize sgRNAs for CRISPR screen experiments. With official gene symbols or RefSeq IDs as the only mandatory input, CRISPR-FOCUS filters and prioritizes sgRNAs based on multiple criteria, including efficiency, specificity, sequence conservation, isoform structure, as well as genomic variations including Single Nucleotide Polymorphisms and cancer somatic mutations. CRISPR-FOCUS also provides pre-defined positive and negative control sgRNAs, as well as other necessary sequences in the construct (e.g., U6 promoters to drive sgRNA transcription and RNA scaffolds of the CRISPR/Cas9). These features allow users to synthesize oligonucleotides directly based on the output of CRISPR-FOCUS. Overall, CRISPR-FOCUS provides a rational and high-throughput approach for sgRNA library design that enables users to efficiently conduct a focused screen experiment targeting up to thousands of genes.(CRISPR-FOCUS is freely available at http://cistrome.org/crispr-focus/) Twit Text FOAVip CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28873439 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28873439 #FOAvip English Wikipedia <ref>{{Cite pmid|28873439}}</ref> CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments #MMPMID28873439 Cao Q; Ma J; Chen CH; Xu H; Chen Z; Li W; Liu XS PLoS One 2017[]; 12 (9): ä PMID28873439show ga The recently developed CRISPR screen technology, based on the CRISPR/Cas9 genome editing system, enables genome-wide interrogation of gene functions in an efficient and cost-effective manner. Although many computational algorithms and web servers have been developed to design single-guide RNAs (sgRNAs) with high specificity and efficiency, algorithms specifically designed for conducting CRISPR screens are still lacking. Here we present CRISPR-FOCUS, a web-based platform to search and prioritize sgRNAs for CRISPR screen experiments. With official gene symbols or RefSeq IDs as the only mandatory input, CRISPR-FOCUS filters and prioritizes sgRNAs based on multiple criteria, including efficiency, specificity, sequence conservation, isoform structure, as well as genomic variations including Single Nucleotide Polymorphisms and cancer somatic mutations. CRISPR-FOCUS also provides pre-defined positive and negative control sgRNAs, as well as other necessary sequences in the construct (e.g., U6 promoters to drive sgRNA transcription and RNA scaffolds of the CRISPR/Cas9). These features allow users to synthesize oligonucleotides directly based on the output of CRISPR-FOCUS. Overall, CRISPR-FOCUS provides a rational and high-throughput approach for sgRNA library design that enables users to efficiently conduct a focused screen experiment targeting up to thousands of genes.(CRISPR-FOCUS is freely available at http://cistrome.org/crispr-focus/) äCRISPR-FOCUS: A web server for designing focused CRISPR screening expe(epmc).pdf DeepDyve Pubget Overpricing