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10.1155/2017/4825108 http://scihub22266oqcxt.onion/10.1155/2017/4825108 C5584363!5584363!28890895     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomed+Res+Int 2017 ; 2017 (ä): ä Nephropedia Template TP {{tp|p=28890895|t=2017. Isolation of Fibroblast-Activation Protein-Specific Cancer-Associated Fibroblasts |pdf=|usr=}}{{28890895}} gab.com Text Isolation of Fibroblast-Activation Protein-Specific Cancer-Associated Fibroblasts JO: Biomed Res Int http://www.kidney.de/pget.php?&tw=1&pmid=28890895 #FOAvip The current study is to develop a gentle and efficient method for purification of fibroblast-activation protein positive (FAP+) cancer-associated fibroblasts (CAFs) from tumor tissues. Fresh tissues were isolated from BALB/c-Nude mice bearing human liver cancer cell line (HepG2), fully minced and separated into three parts, and digested with trypsin digestion and then treated with collagenase type IV once, twice, or thrice, respectively. Finally, the cells were purified by using FAP magnetic beads. The isolated CAFs were grown in culture medium and detected for the surface expression of fibroblast-activation protein (FAP). The number of adherent cells which were obtained by digestion process with twice collagenase type IV digestion was (5.99 ± 0.18) × 104, much more than that with the only once collagenase type IV digestion (2.58 ± 0.41) × 104 (P < 0.0001) and similar to thrice collagenase type IV digestion. The percentage of FAP+ CAFs with twice collagenase type IV digestion (38.5%) was higher than that with the only once collagenase type IV digestion (20.0%) and little higher than thrice collagenase type IV digestion (37.5%). The FAP expression of CAFs was quite different from normal fibroblasts (NFs). The fibroblasts isolated by the innovation are with high purity and being in wonderful condition and display the features of CAFs. Twit Text FOAVip Isolation of Fibroblast-Activation Protein-Specific Cancer-Associated Fibroblasts ????Biomed Res Int http://www.kidney.de/pget.php?&tw=1&pmid=28890895 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28890895 #FOAvip English Wikipedia <ref>{{Cite pmid|28890895}}</ref> Isolation of Fibroblast-Activation Protein-Specific Cancer-Associated Fibroblasts #MMPMID28890895 Huang Y; Zhou S; Huang Y; Zheng D; Mao Q; He J; Wang Y; Xue D; Lu X; Yang N; Zhao Y Biomed Res Int 2017[]; 2017 (ä): ä PMID28890895show ga The current study is to develop a gentle and efficient method for purification of fibroblast-activation protein positive (FAP+) cancer-associated fibroblasts (CAFs) from tumor tissues. Fresh tissues were isolated from BALB/c-Nude mice bearing human liver cancer cell line (HepG2), fully minced and separated into three parts, and digested with trypsin digestion and then treated with collagenase type IV once, twice, or thrice, respectively. Finally, the cells were purified by using FAP magnetic beads. The isolated CAFs were grown in culture medium and detected for the surface expression of fibroblast-activation protein (FAP). The number of adherent cells which were obtained by digestion process with twice collagenase type IV digestion was (5.99 ± 0.18) × 104, much more than that with the only once collagenase type IV digestion (2.58 ± 0.41) × 104 (P < 0.0001) and similar to thrice collagenase type IV digestion. The percentage of FAP+ CAFs with twice collagenase type IV digestion (38.5%) was higher than that with the only once collagenase type IV digestion (20.0%) and little higher than thrice collagenase type IV digestion (37.5%). The FAP expression of CAFs was quite different from normal fibroblasts (NFs). The fibroblasts isolated by the innovation are with high purity and being in wonderful condition and display the features of CAFs. äIsolation of Fibroblast-Activation Protein-Specific Cancer-Associated (epmc).pdf DeepDyve Pubget Overpricing