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2017 ; 8
(32
): 53465-53481
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Differential gene and lncRNA expression in the lower thoracic spinal cord
following ischemia/reperfusion-induced acute kidney injury in rats
#MMPMID28881824
Liu QQ
; Liu H
; He ZG
; Zhang SJ
; Liu BW
; Wang L
; Qiu WH
; Xu Q
; Xiang HB
; Lv YM
Oncotarget
2017[Aug]; 8
(32
): 53465-53481
PMID28881824
show ga
We used high-throughput RNA sequencing to analyze differential gene and lncRNA
expression patterns in the lower thoracic spinal cord during ischemia/reperfusion
(I/R)-induced acute kidney injury (AKI) in rats. We observed that of 32662 mRNAs,
4296 out were differentially expressed in the T8-12 segments of the spinal cord
upon I/R-induced AKI. Among these, 62 were upregulated and 34 were downregulated
in response to I/R (FDR < 0.05, |log(2)FC| > 1). Further, 52 differentially
expressed lncRNAs (35 upregulated and 17 downregulated) were identified among
3849 lncRNA transcripts. The differentially expressed mRNAs were annotated as
"biological process," "cellular components" and "molecular functions" through
gene ontology enrichment analysis. KEGG pathway enrichment analysis showed that
cell cycle and renin-angiotensin pathways were upregulated in response to I/R,
while protein digestion and absorption, hedgehog, neurotrophin, MAPK, and
PI3K-Akt signaling were downregulated. The RNA-seq data was validated by qRT-PCR
and western blot analyses of select mRNAs and lncRNAs. We observed that Bax,
Caspase-3 and phospho-AKT were upregulated and Bcl-2 was downregulated in the
spinal cord in response to renal injury. We also found negative correlations
between three lncRNAs (TCONS_00042175, TCONS_00058568 and TCONS_00047728) and the
degree of renal injury. These findings provide evidence for differential
expression of lncRNAs and mRNAs in the lower thoracic spinal cord following
I/R-induced AKI in rats and suggest potential clinical applicability.
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