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2017 ; 7
(1
): 10297
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Chill coma in the locust, Locusta migratoria, is initiated by spreading
depolarization in the central nervous system
#MMPMID28860653
Robertson RM
; Spong KE
; Srithiphaphirom P
Sci Rep
2017[Aug]; 7
(1
): 10297
PMID28860653
show ga
The ability of chill-sensitive insects to function at low temperatures limits
their geographic ranges. They have species-specific temperatures below which
movements become uncoordinated prior to entering a reversible state of
neuromuscular paralysis. In spite of decades of research, which in recent years
has focused on muscle function, the role of neural mechanisms in determining
chill coma is unknown. Spreading depolarization (SD) is a phenomenon that causes
a shutdown of neural function in the integrating centres of the central nervous
system. We investigated the role of SD in the process of entering chill coma in
the locust, Locusta migratoria. We used thermolimit respirometry and
electromyography in whole animals and extracellular and intracellular recording
techniques in semi-intact preparations to characterize neural events during
chilling. We show that chill-induced SD in the central nervous system is the
mechanism underlying the critical thermal minimum for coordinated movement in
locusts. This finding will be important for understanding how insects adapt and
acclimate to changing environmental temperatures.