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10.1007/s12551-017-0278-2

http://scihub22266oqcxt.onion/10.1007/s12551-017-0278-2
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C5578921!5578921!28752365
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suck abstract from ncbi


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pmid28752365      Biophys+Rev 2017 ; 9 (4): 299-308
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  • Alternative reagents to antibodies in imaging applications #MMPMID28752365
  • Bedford R; Tiede C; Hughes R; Curd A; McPherson MJ; Peckham M; Tomlinson DC
  • Biophys Rev 2017[Aug]; 9 (4): 299-308 PMID28752365show ga
  • Antibodies have been indispensable tools in molecular biology, biochemistry and medical research. However, a number of issues surrounding validation, specificity and batch variation of commercially available antibodies have prompted research groups to develop novel non-antibody binding reagents. The ability to select highly specific monoclonal non-antibody binding proteins without the need for animals, the ease of production and the ability to site-directly label has enabled a wide variety of applications to be tested, including imaging. In this review, we discuss the success of a number of non-antibody reagents in imaging applications, including the recently reported Affimer.
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