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Alternative reagents to antibodies in imaging applications #MMPMID28752365
Bedford R; Tiede C; Hughes R; Curd A; McPherson MJ; Peckham M; Tomlinson DC
Biophys Rev 2017[Aug]; 9 (4): 299-308 PMID28752365show ga
Antibodies have been indispensable tools in molecular biology, biochemistry and medical research. However, a number of issues surrounding validation, specificity and batch variation of commercially available antibodies have prompted research groups to develop novel non-antibody binding reagents. The ability to select highly specific monoclonal non-antibody binding proteins without the need for animals, the ease of production and the ability to site-directly label has enabled a wide variety of applications to be tested, including imaging. In this review, we discuss the success of a number of non-antibody reagents in imaging applications, including the recently reported Affimer.