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2017 ; 18
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): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
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English Wikipedia
Imaging Collagen in Scar Tissue: Developments in Second Harmonic Generation
Microscopy for Biomedical Applications
#MMPMID28809791
Mostaço-Guidolin L
; Rosin NL
; Hackett TL
Int J Mol Sci
2017[Aug]; 18
(8
): ä PMID28809791
show ga
The ability to respond to injury with tissue repair is a fundamental property of
all multicellular organisms. The extracellular matrix (ECM), composed of
fibrillar collagens as well as a number of other components is dis-regulated
during repair in many organs. In many tissues, scaring results when the balance
is lost between ECM synthesis and degradation. Investigating what disrupts this
balance and what effect this can have on tissue function remains an active area
of research. Recent advances in the imaging of fibrillar collagen using second
harmonic generation (SHG) imaging have proven useful in enhancing our
understanding of the supramolecular changes that occur during scar formation and
disease progression. Here, we review the physical properties of SHG, and the
current nonlinear optical microscopy imaging (NLOM) systems that are used for SHG
imaging. We provide an extensive review of studies that have used SHG in skin,
lung, cardiovascular, tendon and ligaments, and eye tissue to understand
alterations in fibrillar collagens in scar tissue. Lastly, we review the current
methods of image analysis that are used to extract important information about
the role of fibrillar collagens in scar formation.