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10.1093/aob/mcx036 http://scihub22266oqcxt.onion/10.1093/aob/mcx036 C5577905!5577905!28444369     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Ann+Bot 2017 ; 120 (1): 21-8 Nephropedia Template TP {{tp|p=28444369|t=2017. Three-dimensional intracellular structure of a whole rice mesophyll cell observed with FIB-SEM |pdf=|usr=}}{{28444369}} gab.com Text Three-dimensional intracellular structure of a whole rice mesophyll cell observed with FIB-SEM JO: Ann Bot http://www.kidney.de/pget.php?&tw=1&pmid=28444369 #FOAvip Background and Aims Ultrathin sections of rice leaf blades observed two-dimensionally using a transmission electron microscope (TEM) show that the chlorenchyma is composed of lobed mesophyll cells, with intricate cell boundaries, and lined with chloroplasts. The lobed cell shape and chloroplast positioning are believed to enhance the area available for the gas exchange surface for photosynthesis in rice leaves. However, a cell image revealing the three-dimensional (3-D) ultrastructure of rice mesophyll cells has not been visualized. In this study, a whole rice mesophyll cell was observed using a focused ion beam scanning electron microscope (FIB-SEM), which provides many serial sections automatically, rapidly and correctly, thereby enabling 3-D cell structure reconstruction.Methods Rice leaf blades were fixed chemically using the method for conventional TEM observation, embedded in resin and subsequently set in the FIB-SEM chamber. Specimen blocks were sectioned transversely using the FIB, and block-face images were captured using the SEM. The sectioning and imaging were repeated overnight for 200?500 slices (each 50?nm thick). The resultant large-volume image stacks (x = 25??m, y = 25??m, z = 10?25??m) contained one or two whole mesophyll cells. The 3-D models of whole mesophyll cells were reconstructed using image processing software.Key Results The reconstructed cell models were discoid shaped with several lobes around the cell periphery. The cell shape increased the surface area, and the ratio of surface area to volume was twice that of a cylinder having the same volume. The chloroplasts occupied half the cell volume and spread as sheets along the cell lobes, covering most of the inner cell surface, with adjacent chloroplasts in close contact with each other.Conclusions Cellular and sub-cellular ultrastructures of a whole mesophyll cell in a rice leaf blade are demonstrated three-dimensionally using a FIB-SEM. The 3-D models and numerical information support the hypothesis that rice mesophyll cells enhance their CO2 absorption with increased cell surface and sheet-shaped chloroplasts. Twit Text FOAVip Three-dimensional intracellular structure of a whole rice mesophyll cell observed with FIB-SEM ????Ann Bot http://www.kidney.de/pget.php?&tw=1&pmid=28444369 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28444369 #FOAvip English Wikipedia <ref>{{Cite pmid|28444369}}</ref> Three-dimensional intracellular structure of a whole rice mesophyll cell observed with FIB-SEM #MMPMID28444369 Oi T; Enomoto S; Nakao T; Arai S; Yamane K; Taniguchi M Ann Bot 2017[Jul]; 120 (1): 21-8 PMID28444369show ga Background and Aims Ultrathin sections of rice leaf blades observed two-dimensionally using a transmission electron microscope (TEM) show that the chlorenchyma is composed of lobed mesophyll cells, with intricate cell boundaries, and lined with chloroplasts. The lobed cell shape and chloroplast positioning are believed to enhance the area available for the gas exchange surface for photosynthesis in rice leaves. However, a cell image revealing the three-dimensional (3-D) ultrastructure of rice mesophyll cells has not been visualized. In this study, a whole rice mesophyll cell was observed using a focused ion beam scanning electron microscope (FIB-SEM), which provides many serial sections automatically, rapidly and correctly, thereby enabling 3-D cell structure reconstruction.Methods Rice leaf blades were fixed chemically using the method for conventional TEM observation, embedded in resin and subsequently set in the FIB-SEM chamber. Specimen blocks were sectioned transversely using the FIB, and block-face images were captured using the SEM. The sectioning and imaging were repeated overnight for 200?500 slices (each 50?nm thick). The resultant large-volume image stacks (x = 25??m, y = 25??m, z = 10?25??m) contained one or two whole mesophyll cells. The 3-D models of whole mesophyll cells were reconstructed using image processing software.Key Results The reconstructed cell models were discoid shaped with several lobes around the cell periphery. The cell shape increased the surface area, and the ratio of surface area to volume was twice that of a cylinder having the same volume. The chloroplasts occupied half the cell volume and spread as sheets along the cell lobes, covering most of the inner cell surface, with adjacent chloroplasts in close contact with each other.Conclusions Cellular and sub-cellular ultrastructures of a whole mesophyll cell in a rice leaf blade are demonstrated three-dimensionally using a FIB-SEM. The 3-D models and numerical information support the hypothesis that rice mesophyll cells enhance their CO2 absorption with increased cell surface and sheet-shaped chloroplasts. äThree-dimensional intracellular structure of a whole rice mesophyll ce(epmc).pdf DeepDyve Pubget Overpricing