Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28855631
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28855631
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Sci+Rep
2017 ; 7
(1
): 9942
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Functional analysis of a novel, thyroglobulin-embedded microRNA gene deregulated
in papillary thyroid carcinoma
#MMPMID28855631
Kolanowska M
; Wójcicka A
; Kubiak A
; ?wierniak M
; Kotlarek M
; Maci?g M
; Gaj P
; Koperski ?
; Górnicka B
; Ja?d?ewski K
Sci Rep
2017[Aug]; 7
(1
): 9942
PMID28855631
show ga
MicroRNAs, non-coding regulators of gene expression, are known culprits of
thyroid cancer. Using next-generation sequencing, we identified a novel microRNA
gene, encoded within an important thyroid regulator - thyroglobulin, and analyzed
its functionality in the thyroid gland. In vitro and in silico analyses proved
that the novel miR-TG is processed from the precursor, and co-expressed with
thyroglobulin. Both genes are specific for thyroid tissue and downregulated in
papillary thyroid carcinoma by 44% (p?=?0.04) and 48% (p?=?0.001), respectively.
Putative target genes for miR-TG were identified using in silico tools, which
pinpointed MAP4K4, an oncogene upregulated in thyroid cancer. Analysis of
transcriptome by RNA-seq revealed that overexpression of miR-TG in PTC-derived
cell line led to downregulation of several genes, including MAP4K4 (fold change
0,82; p?=?0.036). The finding was confirmed by SQ-PCR (fold change 071;
p?=?0.004). Direct interaction between miR-TG and MAP4K4 was confirmed in the
luciferase assay (p?=?0.0006). Functional studies showed increase proliferation
in K1 cell line transfected with miR-TG. We propose that in normal thyroid miR-TG
plays a fine-tuning effect on the maintenance of MAPK pathway, inhibiting the
expression of miR's target MAP4K4. This regulation is disturbed in cancer due to
downregulation of the novel, thyroglobulin-embedded microRNA, characterized in
this study.
free
free
free
