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2017 ; 46
(14
): 4245-4280
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Materials and microfluidics: enabling the efficient isolation and analysis of
circulating tumour cells
#MMPMID28632258
Jackson JM
; Witek MA
; Kamande JW
; Soper SA
Chem Soc Rev
2017[Jul]; 46
(14
): 4245-4280
PMID28632258
show ga
We present a critical review of microfluidic technologies and material effects on
the analyses of circulating tumour cells (CTCs) selected from the peripheral
blood of cancer patients. CTCs are a minimally invasive source of clinical
information that can be used to prognose patient outcome, monitor minimal
residual disease, assess tumour resistance to therapeutic agents, and potentially
screen individuals for the early diagnosis of cancer. The performance of CTC
isolation technologies depends on microfluidic architectures, the underlying
principles of isolation, and the choice of materials. We present a critical
review of the fundamental principles used in these technologies and discuss their
performance. We also give context to how CTC isolation technologies enable
downstream analysis of selected CTCs in terms of detecting genetic mutations and
gene expression that could be used to gain information that may affect patient
outcome.