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10.1242/bio.025320 http://scihub22266oqcxt.onion/10.1242/bio.025320 C5576077!5576077!28663236     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biol+Open 2017 ; 6 (8): 1245-56 Nephropedia Template TP {{tp|p=28663236|t=2017. Manipulation and quantification of microtubule lattice integrity |pdf=|usr=}}{{28663236}} gab.com Text Manipulation and quantification of microtubule lattice integrity JO: Biol Open http://www.kidney.de/pget.php?&tw=1&pmid=28663236 #FOAvip Microtubules are structural polymers that participate in a wide range of cellular functions. The addition and loss of tubulin subunits allows the microtubule to grow and shorten, as well as to develop and repair defects and gaps in its cylindrical lattice. These lattice defects act to modulate the interactions of microtubules with molecular motors and other microtubule-associated proteins. Therefore, tools to control and measure microtubule lattice structure will be invaluable for developing a quantitative understanding of how the structural state of the microtubule lattice may regulate its interactions with other proteins. In this work, we manipulated the lattice integrity of in vitro microtubules to create pools of microtubules with common nucleotide states, but with variations in structural states. We then developed a series of novel semi-automated analysis tools for both fluorescence and electron microscopy experiments to quantify the type and severity of alterations in microtubule lattice integrity. These techniques will enable new investigations that explore the role of microtubule lattice structure in interactions with microtubule-associated proteins. Twit Text FOAVip Manipulation and quantification of microtubule lattice integrity ????Biol Open http://www.kidney.de/pget.php?&tw=1&pmid=28663236 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28663236 #FOAvip English Wikipedia <ref>{{Cite pmid|28663236}}</ref> Manipulation and quantification of microtubule lattice integrity #MMPMID28663236 Reid TA; Coombes C; Gardner MK Biol Open 2017[Aug]; 6 (8): 1245-56 PMID28663236show ga Microtubules are structural polymers that participate in a wide range of cellular functions. The addition and loss of tubulin subunits allows the microtubule to grow and shorten, as well as to develop and repair defects and gaps in its cylindrical lattice. These lattice defects act to modulate the interactions of microtubules with molecular motors and other microtubule-associated proteins. Therefore, tools to control and measure microtubule lattice structure will be invaluable for developing a quantitative understanding of how the structural state of the microtubule lattice may regulate its interactions with other proteins. In this work, we manipulated the lattice integrity of in vitro microtubules to create pools of microtubules with common nucleotide states, but with variations in structural states. We then developed a series of novel semi-automated analysis tools for both fluorescence and electron microscopy experiments to quantify the type and severity of alterations in microtubule lattice integrity. These techniques will enable new investigations that explore the role of microtubule lattice structure in interactions with microtubule-associated proteins. äManipulation and quantification of microtubule lattice integrity (epmc).pdf DeepDyve Pubget Overpricing