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10.1002/jcp.25935 http://scihub22266oqcxt.onion/10.1002/jcp.25935 C5575563!5575563 !28369848     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 296.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 296.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28369848 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Cell+Physiol 2017 ; 232 (11 ): 2985-2995 Nephropedia Template TP {{tp|p=28369848 |t=2017. End stage renal disease-induced hypercalcemia may promote aortic valve calcification via Annexin VI enrichment of valve interstitial cell derived-matrix vesicles |pdf=|usr=}}{{28369848 }} gab.com Text End stage renal disease-induced hypercalcemia may promote aortic valve calcification via Annexin VI enrichment of valve interstitial cell derived-matrix vesicles JO: J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=28369848 #FOAvip Patients with end-stage renal disease (ESRD) have elevated circulating calcium (Ca) and phosphate (Pi), and exhibit accelerated progression of calcific aortic valve disease (CAVD). We hypothesized that matrix vesicles (MVs) initiate the calcification process in CAVD. Ca induced rat valve interstitial cells (VICs) calcification at 4.5?mM (16.4-fold; p?<?0.05) whereas Pi treatment alone had no effect. Ca (2.7?mM) and Pi (2.5?mM) synergistically induced calcium deposition (10.8-fold; p?<?0.001) in VICs. Ca treatment increased the mRNA of the osteogenic markers Msx2, Runx2, and Alpl (p?<?0.01). MVs were harvested by ultracentrifugation from VICs cultured with control or calcification media (containing 2.7?mM Ca and 2.5?mM Pi) for 16?hr. Proteomics analysis revealed the marked enrichment of exosomal proteins, including CD9, CD63, LAMP-1, and LAMP-2 and a concomitant up-regulation of the Annexin family of calcium-binding proteins. Of particular note Annexin VI was shown to be enriched in calcifying VIC-derived MVs (51.9-fold; p?<?0.05). Through bioinformatic analysis using Ingenuity Pathway Analysis (IPA), the up-regulation of canonical signaling pathways relevant to cardiovascular function were identified in calcifying VIC-derived MVs, including aldosterone, Rho kinase, and metal binding. Further studies using human calcified valve tissue revealed the co-localization of Annexin VI with areas of MVs in the extracellular matrix by transmission electron microscopy (TEM). Together these findings highlight a critical role for VIC-derived MVs in CAVD. Furthermore, we identify calcium as a key driver of aortic valve calcification, which may directly underpin the increased susceptibility of ESRD patients to accelerated development of CAVD. Twit Text FOAVip End stage renal disease-induced hypercalcemia may promote aortic valve calcification via Annexin VI enrichment of valve interstitial cell derived-matrix vesicles ????J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=28369848 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28369848 #FOAvip English Wikipedia <ref>{{Cite pmid|28369848 }}</ref> End stage renal disease-induced hypercalcemia may promote aortic valve calcification via Annexin VI enrichment of valve interstitial cell derived-matrix vesicles #MMPMID28369848 Cui L ; Rashdan NA ; Zhu D ; Milne EM ; Ajuh P ; Milne G ; Helfrich MH ; Lim K ; Prasad S ; Lerman DA ; Vesey AT ; Dweck MR ; Jenkins WS ; Newby DE ; Farquharson C ; Macrae VE J Cell Physiol 2017[Nov]; 232 (11 ): 2985-2995 PMID28369848 show ga Patients with end-stage renal disease (ESRD) have elevated circulating calcium (Ca) and phosphate (Pi), and exhibit accelerated progression of calcific aortic valve disease (CAVD). We hypothesized that matrix vesicles (MVs) initiate the calcification process in CAVD. Ca induced rat valve interstitial cells (VICs) calcification at 4.5?mM (16.4-fold; p? |Aged [MESH] |Alkaline Phosphatase/genetics/metabolism [MESH] |Animals [MESH] |Annexin A6/*metabolism [MESH] |Aortic Valve Stenosis/etiology/genetics/*metabolism/pathology [MESH] |Aortic Valve/*metabolism/*pathology/ultrastructure [MESH] |Calcinosis/etiology/genetics/*metabolism/pathology [MESH] |Calcium/metabolism [MESH] |Cells, Cultured [MESH] |Core Binding Factor Alpha 1 Subunit/genetics/metabolism [MESH] |Extracellular Matrix/*metabolism/ultrastructure [MESH] |Extracellular Vesicles/*metabolism/ultrastructure [MESH] |Female [MESH] |Homeodomain Proteins/genetics/metabolism [MESH] |Humans [MESH] |Hypercalcemia/diagnosis/*etiology [MESH] |Kidney Failure, Chronic/*complications/diagnosis [MESH] |Male [MESH] |Microscopy, Electron, Transmission [MESH] |Protein Interaction Maps [MESH] |Proteomics/methods [MESH] |RNA, Messenger/genetics/metabolism [MESH] |Rats, Sprague-Dawley [MESH]End stage renal disease-induced hypercalcemia may promote aortic valve(epmc).pdf DeepDyve Pubget Overpricing