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10.1038/s41598-017-09761-6 http://scihub22266oqcxt.onion/10.1038/s41598-017-09761-6 C5575107!5575107 !28851970     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28851970 &cmd=llinks): Failed to open stream: HTTP request failed! 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Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading |pdf=|usr=}}{{28851970 }} gab.com Text Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28851970 #FOAvip Dramatic and rapid changes in cell shape are perhaps best exemplified by phagocytes, such as neutrophils. These cells complete the processes of spreading onto surfaces, and phagocytosis within 100?s of stimulation. Although these cell shape changes are accompanied by an apparent large increase in cell surface area, the nature of the membrane "reservoir" for the additional area is unclear. One proposal is that the wrinkled cell surface topography (which forms micro-ridges on the neutrophil surface) provides the resource for neutrophils to expand their available surface area. However, it has been problematic to test this proposal in living cells because these surface structures are sub-light microscopic. In this paper, we report the development of a novel approach, a variant of FRAP (fluorescent recovery after photo-bleaching) modified to interrogate the diffusion path-lengths of membrane associated molecules. This approach provides clear evidence that the cell surface topography changes dramatically during neutrophil shape change (both locally and globally) and can be triggered by elevating cytosolic Ca(2+). Twit Text FOAVip Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28851970 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28851970 #FOAvip English Wikipedia <ref>{{Cite pmid|28851970 }}</ref> Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading #MMPMID28851970 Jumaa MAA ; Dewitt S ; Hallett MB Sci Rep 2017[Aug]; 7 (1 ): 9790 PMID28851970 show ga Dramatic and rapid changes in cell shape are perhaps best exemplified by phagocytes, such as neutrophils. These cells complete the processes of spreading onto surfaces, and phagocytosis within 100?s of stimulation. Although these cell shape changes are accompanied by an apparent large increase in cell surface area, the nature of the membrane "reservoir" for the additional area is unclear. One proposal is that the wrinkled cell surface topography (which forms micro-ridges on the neutrophil surface) provides the resource for neutrophils to expand their available surface area. However, it has been problematic to test this proposal in living cells because these surface structures are sub-light microscopic. In this paper, we report the development of a novel approach, a variant of FRAP (fluorescent recovery after photo-bleaching) modified to interrogate the diffusion path-lengths of membrane associated molecules. This approach provides clear evidence that the cell surface topography changes dramatically during neutrophil shape change (both locally and globally) and can be triggered by elevating cytosolic Ca(2+). |*Cell Movement [MESH] |*Cell Shape [MESH] |*Phagocytosis [MESH] |Biomarkers [MESH] |Calcium/metabolism [MESH] |Fluorescent Antibody Technique [MESH] |Humans [MESH] |Microscopy, Electron, Scanning [MESH] |Microscopy, Fluorescence [MESH] |Neutrophils/cytology/physiology [MESH]Topographical interrogation of the living cell surface reveals its rol(epmc).pdf DeepDyve Pubget Overpricing