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10.3389/fimmu.2017.01030 http://scihub22266oqcxt.onion/10.3389/fimmu.2017.01030 C5573807!5573807!28883823     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Immunol 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=28883823|t=2017. Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy |pdf=|usr=}}{{28883823}} gab.com Text Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy JO: Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=28883823 #FOAvip Single-domain antibodies (sdAbs) have substantially expanded the possibilities of advanced cellular imaging such as live-cell or super-resolution microscopy to visualize cellular antigens and their dynamics. In addition to their unique properties including small size, high stability, and solubility in many environments, sdAbs can be efficiently functionalized according to the needs of the respective imaging approach. Genetically encoded intrabodies fused to fluorescent proteins (chromobodies) have become versatile tools to study dynamics of endogenous proteins in living cells. Additionally, sdAbs conjugated to organic dyes were shown to label cellular structures with high density and minimal fluorophore displacement making them highly attractive probes for super-resolution microscopy. Here, we review recent advances of the chromobody technology to visualize localization and dynamics of cellular targets and the application of chromobody-based cell models for compound screening. Acknowledging the emerging importance of super-resolution microscopy in cell biology, we further discuss advantages and challenges of sdAbs for this technology. Twit Text FOAVip Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy ????Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=28883823 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28883823 #FOAvip English Wikipedia <ref>{{Cite pmid|28883823}}</ref> Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy #MMPMID28883823 Traenkle B; Rothbauer U Front Immunol 2017[]; 8 (ä): ä PMID28883823show ga Single-domain antibodies (sdAbs) have substantially expanded the possibilities of advanced cellular imaging such as live-cell or super-resolution microscopy to visualize cellular antigens and their dynamics. In addition to their unique properties including small size, high stability, and solubility in many environments, sdAbs can be efficiently functionalized according to the needs of the respective imaging approach. Genetically encoded intrabodies fused to fluorescent proteins (chromobodies) have become versatile tools to study dynamics of endogenous proteins in living cells. Additionally, sdAbs conjugated to organic dyes were shown to label cellular structures with high density and minimal fluorophore displacement making them highly attractive probes for super-resolution microscopy. Here, we review recent advances of the chromobody technology to visualize localization and dynamics of cellular targets and the application of chromobody-based cell models for compound screening. Acknowledging the emerging importance of super-resolution microscopy in cell biology, we further discuss advantages and challenges of sdAbs for this technology. äUnder the Microscope: Single-Domain Antibodies for Live-Cell Imaging a(epmc).pdf DeepDyve Pubget Overpricing