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2017 ; 8
(ä): 1569
Nephropedia Template TP
gab.com Text
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Twit Text #
English Wikipedia
From Farms to Markets: Gram-Negative Bacteria Resistant to Third-Generation
Cephalosporins in Fruits and Vegetables in a Region of North Africa
#MMPMID28883810
Mesbah Zekar F
; Granier SA
; Marault M
; Yaici L
; Gassilloud B
; Manceau C
; Touati A
; Millemann Y
Front Microbiol
2017[]; 8
(ä): 1569
PMID28883810
show ga
The role of food in human exposure to antimicrobial-resistant bacteria is a
growing food safety issue. The contribution of fruits and vegetables eaten raw to
this exposure is still unclear. The evaluation of contamination levels of fruits,
vegetables and the agricultural environment by third-generation cephalosporin
(3GC)-resistant Gram-negative bacteria was performed by analyzing 491 samples of
fruits and vegetables collected from 5 markets and 7 farms in Bejaia area,
north-eastern Mediterranean coast of Algeria. Ninety soil samples and 45
irrigation water samples were also sampled in farms in order to assess them as
potential inoculum sources. All samples were investigated at the same time on
ceftazidime-containing selective media for 3GC-resistant Gram-negative bacteria
detection and on Hektoen media, for Salmonella spp. presence. The bacteria
isolated (n = 30) from fruits and vegetables, soil and irrigation water collected
in the farms were almost all non-fermenting bacterial species (Stenotrophomonas,
Acinetobacter, Pseudomonas, Ochrobactrum) except one strain of Enterobacter
cloacae and two strains of Citrobacter murliniae, isolated on one cucumber and
two tomato samples in the same farm. Greater diversity in bacterial species and
antimicrobial resistance profiles was observed at markets: Enterobacteriaceae (n
= 41) were as strongly represented as non-fermenting bacteria (n = 37). Among
Enterobacteriaceae, E. cloacae (n = 21), and Klebsiella pneumoniae (n = 13) were
the most common isolates. Most of the K. pneumoniae isolates were
extended-spectrum beta-lactamase (ESBL) producers (n = 11). No Salmonella spp.
was recovered in any sample. This study showed that fruits and vegetables
including those which may be eaten up raw constitute a reservoir of 3GC-resistant
Gram-negative bacteria and multi-drug resistant-bacteria in general that can be
transferred to humans through food. The general public should be informed of this
hazard for health in order to encourage good domestic hygiene practices. In
addition, further investigation is needed throughout the production chain to
enrol professionals in actions to reduce this contamination.