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2017 ; 12
(8
): e0183399
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Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using
coherence-controlled holographic microscopy
#MMPMID28846747
Gál B
; Veselý M
; ?olláková J
; Nekulová M
; J?zová V
; Chmelík R
; Veselý P
PLoS One
2017[]; 12
(8
): e0183399
PMID28846747
show ga
Head and neck squamous cell carcinoma is one of the most aggressive tumours and
is typically diagnosed too late. Late diagnosis requires an urgent decision on an
effective therapy. An individualized test of chemosensitivity should quickly
indicate the suitability of chemotherapy and radiotherapy. No ex vivo
chemosensitivity assessment developed thus far has become a part of general
clinical practice. Therefore, we attempted to explore the new technique of
coherence-controlled holographic microscopy to investigate the motility and
growth of live cells from a head and neck squamous cell carcinoma biopsy. We
expected to reveal behavioural patterns characteristic for malignant cells that
can be used to imrove future predictive evaluation of chemotherapy. We managed to
cultivate primary SACR2 carcinoma cells from head and neck squamous cell
carcinoma biopsy verified through histopathology. The cells grew as a cohesive
sheet of suspected carcinoma origin, and western blots showed positivity for the
tumour marker p63 confirming cancerous origin. Unlike the roundish colonies of
the established FaDu carcinoma cell line, the SACR2 cells formed irregularly
shaped colonies, eliciting the impression of the collective invasion of carcinoma
cells. Time-lapse recordings of the cohesive sheet activity revealed the rapid
migration and high plasticity of these epithelial-like cells. Individual cells
frequently abandoned the swiftly migrating crowd by moving aside and crawling
faster. The increasing mass of fast migrating epithelial-like cells before and
after mitosis confirmed the continuation of the cell cycle. In
immunofluorescence, analogously shaped cells expressed the p63 tumour marker,
considered proof of their origin from a carcinoma. These behavioural traits
indicate the feasible identification of carcinoma cells in culture according to
the proposed concept of the carcinoma cell dynamic phenotype. If further
developed, this approach could later serve in a new functional online analysis of
reactions of carcinoma cells to therapy. Such efforts conform to current trends
in precision medicine.
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