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10.1038/s41598-017-08982-z http://scihub22266oqcxt.onion/10.1038/s41598-017-08982-z C5567377!5567377 !28831080     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28831080 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2017 ; 7 (1 ): 9049 Nephropedia Template TP {{tp|p=28831080 |t=2017. Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling |pdf=|usr=}}{{28831080 }} gab.com Text Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28831080 #FOAvip Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression in individual cells to be followed over time using confocal microscopy, while photoacoustic microscopy enables dynamic measurement of blood flow across the network with capillary-level resolution. We combined these two non-invasive imaging modalities in order to spatially and temporally analyze biochemical and biomechanical drivers of angiogenesis in murine corneal neovessels. By stimulating corneal angiogenesis with an alkali burn in Tie2-GFP fluorescent-reporter mice, we evaluated how onset of blood flow and surgically-altered blood flow affects Tie2-GFP expression. Our study establishes a novel platform for analyzing heterogeneous blood flow and fluorescent reporter protein expression across a dynamic microvascular network in an adult mammal. Twit Text FOAVip Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28831080 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28831080 #FOAvip English Wikipedia <ref>{{Cite pmid|28831080 }}</ref> Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling #MMPMID28831080 Kelly-Goss MR ; Ning B ; Bruce AC ; Tavakol DN ; Yi D ; Hu S ; Yates PA ; Peirce SM Sci Rep 2017[Aug]; 7 (1 ): 9049 PMID28831080 show ga Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression in individual cells to be followed over time using confocal microscopy, while photoacoustic microscopy enables dynamic measurement of blood flow across the network with capillary-level resolution. We combined these two non-invasive imaging modalities in order to spatially and temporally analyze biochemical and biomechanical drivers of angiogenesis in murine corneal neovessels. By stimulating corneal angiogenesis with an alkali burn in Tie2-GFP fluorescent-reporter mice, we evaluated how onset of blood flow and surgically-altered blood flow affects Tie2-GFP expression. Our study establishes a novel platform for analyzing heterogeneous blood flow and fluorescent reporter protein expression across a dynamic microvascular network in an adult mammal. |*Gene Expression [MESH] |*Microcirculation [MESH] |Animals [MESH] |Biomarkers [MESH] |Capillaries/*physiology [MESH] |Corneal Neovascularization/genetics/metabolism [MESH] |Endothelial Cells/metabolism [MESH] |Endothelium, Vascular/*metabolism [MESH] |Genes, Reporter [MESH] |Hemodynamics [MESH] |Mice [MESH] |Microscopy, Fluorescence [MESH] |Molecular Imaging [MESH] |Receptor, TIE-2/*genetics [MESH] |Regional Blood Flow/*genetics [MESH]Dynamic, heterogeneous endothelial Tie2 expression and capillary blood(epmc).pdf DeepDyve Pubget Overpricing