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10.1038/s41598-017-08641-3 http://scihub22266oqcxt.onion/10.1038/s41598-017-08641-3 C5567192!5567192 !28827777     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28827777 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2017 ; 7 (1 ): 8360 Nephropedia Template TP {{tp|p=28827777 |t=2017. Efficient generation of bispecific IgG antibodies by split intein mediated protein trans-splicing system |pdf=|usr=}}{{28827777 }} gab.com Text Efficient generation of bispecific IgG antibodies by split intein mediated protein trans-splicing system JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28827777 #FOAvip Many methods have been developed to produce bispecific antibodies (BsAbs) for industrial application. However, huge challenges still remain in synthesizing whole length BsAbs, including their assembly, stability, immunogenicity, and pharmacodynamics. Here we present for first time a generic technology platform of generating bispecific IgG antibodies, "Bispecific Antibody by Protein Trans-splicing (BAPTS)". Different from published methods, we assembled two parental antibody fragments in the hinge region by the protein trans-splicing reaction of a split intein to generate BsAbs without heavy/heavy and light/heavy chain mispairing. Utilizing this simple and efficient approach, there have been several BsAbs (CD3×HER2, CD3×EGFR, EGFR×HER2) synthesized to demonstrate its broad applicability. Correctly paired mAb arms were assembled to form BsAbs that were purified through protein A affinity chromatography to demonstrate industrial applicability at large scale. Further, the products were characterized through physical-biochemistry properties and biological activities to confirm expected quality of the products from "BAPTS". More importantly, correct pairing was confirmed by mass spectrum. Proof-of-concept studies with CD3×HER2 BsAb (T-cell recruitment) demonstrated superior bioactivity compared with trastuzumab. The results of undetectable mispairing and high biological activity have indicated that this method has the potential to be utilized to manufacture BsAbs with high efficiency at industrial scale. Twit Text FOAVip Efficient generation of bispecific IgG antibodies by split intein mediated protein trans-splicing system ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28827777 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28827777 #FOAvip English Wikipedia <ref>{{Cite pmid|28827777 }}</ref> Efficient generation of bispecific IgG antibodies by split intein mediated protein trans-splicing system #MMPMID28827777 Han L ; Chen J ; Ding K ; Zong H ; Xie Y ; Jiang H ; Zhang B ; Lu H ; Yin W ; Gilly J ; Zhu J Sci Rep 2017[Aug]; 7 (1 ): 8360 PMID28827777 show ga Many methods have been developed to produce bispecific antibodies (BsAbs) for industrial application. However, huge challenges still remain in synthesizing whole length BsAbs, including their assembly, stability, immunogenicity, and pharmacodynamics. Here we present for first time a generic technology platform of generating bispecific IgG antibodies, "Bispecific Antibody by Protein Trans-splicing (BAPTS)". Different from published methods, we assembled two parental antibody fragments in the hinge region by the protein trans-splicing reaction of a split intein to generate BsAbs without heavy/heavy and light/heavy chain mispairing. Utilizing this simple and efficient approach, there have been several BsAbs (CD3×HER2, CD3×EGFR, EGFR×HER2) synthesized to demonstrate its broad applicability. Correctly paired mAb arms were assembled to form BsAbs that were purified through protein A affinity chromatography to demonstrate industrial applicability at large scale. Further, the products were characterized through physical-biochemistry properties and biological activities to confirm expected quality of the products from "BAPTS". More importantly, correct pairing was confirmed by mass spectrum. Proof-of-concept studies with CD3×HER2 BsAb (T-cell recruitment) demonstrated superior bioactivity compared with trastuzumab. The results of undetectable mispairing and high biological activity have indicated that this method has the potential to be utilized to manufacture BsAbs with high efficiency at industrial scale. |*Protein Splicing [MESH] |*Trans-Splicing [MESH] |Animals [MESH] |Antibodies, Bispecific/*biosynthesis/isolation & purification/pharmacology [MESH] |CD3 Complex/immunology [MESH] |Cell Proliferation/*drug effects [MESH] |ErbB Receptors/immunology [MESH] |Female [MESH] |Humans [MESH] |Immunoglobulin G/*immunology [MESH] |Mice [MESH] |Mice, Inbred NOD [MESH] |Mice, SCID [MESH] |Protein Engineering/*methods [MESH] |Receptor, ErbB-2/immunology [MESH] |Stomach Neoplasms/drug therapy/immunology/*pathology [MESH] |Tumor Cells, Cultured [MESH]Efficient generation of bispecific IgG antibodies by split intein medi(epmc).pdf DeepDyve Pubget Overpricing