Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1007/s00216-016-9781-8 http://scihub22266oqcxt.onion/10.1007/s00216-016-9781-8 C5566169!5566169!27613013     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Bioanal+Chem 2016 ; 408 (25): 6885-911 Nephropedia Template TP {{tp|p=27613013|t=2016. From single molecules to life: microscopy at the nanoscale |pdf=|usr=}}{{27613013}} gab.com Text From single molecules to life: microscopy at the nanoscale JO: Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=27613013 #FOAvip Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Since their conception a little over a decade ago, these techniques have quickly become the method of choice for many biologists studying structures and processes of single cells at the nanoscale. In this review, we present the three main approaches used to tackle the diffraction barrier of ?200 nm: stimulated-emission depletion (STED) microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy (SMLM). We first present a theoretical overview of the techniques and underlying physics, followed by a practical guide to all of the facets involved in designing a super-resolution experiment, including an approachable explanation of the photochemistry involved, labeling methods available, and sample preparation procedures. Finally, we highlight some of the most exciting recent applications of and developments in these techniques, and discuss the outlook for this field.Graphical AbstractSuper-resolution microscopy techniques. Working principles of the common approaches stimulated-emission depletion (STED) microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy (SMLM). Twit Text FOAVip From single molecules to life: microscopy at the nanoscale ????Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=27613013 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27613013 #FOAvip English Wikipedia <ref>{{Cite pmid|27613013}}</ref> From single molecules to life: microscopy at the nanoscale #MMPMID27613013 Turkowyd B; Virant D; Endesfelder U Anal Bioanal Chem 2016[]; 408 (25): 6885-911 PMID27613013show ga Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Since their conception a little over a decade ago, these techniques have quickly become the method of choice for many biologists studying structures and processes of single cells at the nanoscale. In this review, we present the three main approaches used to tackle the diffraction barrier of ?200 nm: stimulated-emission depletion (STED) microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy (SMLM). We first present a theoretical overview of the techniques and underlying physics, followed by a practical guide to all of the facets involved in designing a super-resolution experiment, including an approachable explanation of the photochemistry involved, labeling methods available, and sample preparation procedures. Finally, we highlight some of the most exciting recent applications of and developments in these techniques, and discuss the outlook for this field.Graphical AbstractSuper-resolution microscopy techniques. Working principles of the common approaches stimulated-emission depletion (STED) microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy (SMLM). äFrom single molecules to life: microscopy at the nanoscale (epmc).pdf DeepDyve Pubget Overpricing